摘要
超薄切片观察云南烟草丛枝症病害的病叶组织，并抽提试验材料的总核酸电泳检测，结果表明：病组织中检测到与烟草丛枝症病害相关的低分子量ＲＮＡ，切片中未观察到病毒粒子，薄壁细胞中发现细胞膜系统的畸变：质膜体（ｐｌａｍａｌｅｍｍａｓｏｍｅ）和双层膜泡囊（ｄｏｕｂｌｅｍｅｍｂｒａｎｏｕｓｖｅｓｉｃｌｅｓ）．质膜体由细胞质膜或液泡膜伸入空泡形成，直径约１０００～２０００ｎｍ，结构类似于类病毒侵染寄主形成的旁壁体．双层膜泡囊是含纤维状物内含物的泡囊，直径约１００～２００ｎｍ，结构类似于某些病毒侵染寄主形成的细胞质泡囊（ｃｙｔｏｐｌａｓｍｉｃｖｅｓｉｃｌｅｓ）和植原体（ｐｈｙｔｏｐｌａｓｍａｓ），但因其广泛分布于寄主细胞中，不能认为是植原体．文献显示这些病变是类病毒和某些病毒侵染的特征，初步认为云南烟草丛枝症病害可能是病毒病害，发病早期细胞内出现小分子核酸侵染的细胞病变特征，此与莫笑晗等发现病株体内含有大量低分子量ＲＮＡ可能有密切关系．
Leaves of Tobacco witches'broom
symptom diseases in Yunnan from diseased plants (detected the Tobacco witches'broom
symptom diseases associated-RNA) and healthy plants were fixed and processed for
transmission electron microscopy.No virus-like and phytoplasmas were found in diseased thin
sections.Aberration of the plasmamenbrane of campanion cells have been identified:the
plamalemmasome and cytoplasmic vesicles,The plamalemmasome (with diameter ranged 1
0002 000 nm) structure likes viroid-associated plamalemmasome,and cytoplasmic vesicles
(with diameter ranged 100200 nm) were double membranous vesicles concluding fibrous thing.
The vesicles were different from phytoplasma in distributing.According to some
documents,these aberrations were the characteristics of the viroid or virus infected leaf tissue.
We think that perhaps the pathogen of the disease is virus.
出处
《云南农业大学学报》
CAS
CSCD
1999年第1期91-95,共5页
Journal of Yunnan Agricultural University
基金
云南省应用基础研究基金
云南省烟草科研基金
关键词
烟草丛枝症病害
超薄切片
细胞质泡囊
质膜体
Tobacco witches'broom
symptom diseases
Ultrastructure
Plamalemmasome
Cytoplasmic vesicles
