摘要
通过蛭石盆栽试验,研究了氮素不同形态配比对菜用大豆[Glycine max (L.) Merr.]品种"理想95-1"生长、种子抗氧化酶活性及活性氧代谢的影响。结果表明,营养液中适宜的硝铵比(75∶25)有利于菜用大豆的生长发育,植株具有最大生物量;在高比例的硝态氮(100%)和铵态氮(75%)处理下,植株的干重、鲜重及产量均显著降低,以硝铵比为25∶75处理下尤为显著。在适宜的硝铵比(75∶25和50∶50)处理下,菜用大豆种子具有较低的抗氧化酶活性,活性氧代谢产物O2、过氧化氢(H2O2)和膜脂过氧化产物丙二醛(MDA)含量也较低,表明植株受到的氧化胁迫程度较低;而在硝铵比为25∶75处理中,抗氧化酶活性最高,O2生成速率、H2O2和MDA含量也最高,表明过多的铵态氮对细胞膜造成了伤害,所受的氧化损伤程度较重。
Using the vermiculite culture,the effects of ratios of NO-3-N and NH＋4-N on plant growth,seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean [Glycine max（L.） Merr.cv.Li-xiang 95-1] were studied.The results show that the appropriate ratio of NO-3-N and NH＋4-N is about 75∶25 which is beneficial to the growth and development of the soybean,and produces the maximum plant biomass.Under the treatment of excessive NO-3（100%） or NH＋4（75%）,both biomass production and yields are decreased obviously,especially for the NH＋4（75%） treatment.In the NO-3∶NH＋4 treatments of 75∶25 and 50∶50, the activities of antioxidant enzymes are low,and the O2 producing rate,hydrogen peroxide（H2O2） and malondiadehyde（MDA） contents are also low,therefore the degree of oxidative stress is comparatively low.However,under the NO3∶NH＋4 treatment of 25∶75,the antioxidant enzyme activities,the O2 producing rate,H2O2 and MDA contents reach to their highest values.These results indicate that excessive NH＋4 is harmful to cell membrane integrity,resulting in severe degree of oxidative damage in the seeds of vegetable soybean.
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2010年第3期768-772,共5页
Journal of Plant Nutrition and Fertilizers
基金
"十一.五"国家高技术(863)研究计划重大项目(编号2006AA10A110)资助
关键词
氮素形态
菜用大豆
抗氧化酶
膜脂过氧化
nitrogen forms
vegetable soybean
antioxidant enzyme activity
membrane lipid peroxidation
