摘要
目的观察天然抗氧化剂茶多酚（Ｔｅａｐｏｌｙｐｈｅｎｏｌｓ，ＴＰ）对重复＋１０Ｇｚ暴露大鼠心功能和心肌超微结构的影响。方法２４只雄性Ｗｉｓｔａｒ大鼠随机等分为三组（ｎ＝８）：Ａ组（对照组），Ｂ组（＋１０Ｇｚ组），Ｃ组（ＴＰ组）。Ｂ、Ｃ组受重复＋１０Ｇｚ作用（每次峰值３０ｓ，Ｇ增长率约为０．５Ｇ／ｓ，３次／ｄ，间隔＋１Ｇｚ／ｍｉｎ，３ｄ／ｗｋ，共４ｗｋ），而Ａ组仅受＋１Ｇｚ作用。Ｃ组于＋Ｇｚ实验前约１ｈ灌胃给予ＴＰ（２００ｍｇ．ｋｇ－１），另两组给予等量蒸馏水。测定离体大鼠工作心脏的心功能并用电镜观察心肌超微结构的变化。结果与对照组相比，重复＋１０Ｇｚ暴露使大鼠左室内压峰值（ＬＶＳＰ）明显降低，心肌细胞超微结构出现明显损伤；而ＴＰ可显著提高＋１０Ｇｚ应激大鼠的ＬＶＳＰ并使心肌超微结构损伤明显减轻。结论重复高Ｇ作用可致大鼠心功能和结构损伤，其作用机理可能部分与自由基代谢有关；而天然抗氧化剂ＴＰ对此具有明显防护作用。
Objective To observe the
effects of tea polyphenols (TP) on cardiac function and myocardial ultrastructure in rats after
repeated +10 Gz stress. Method Twenty four male Wistar rats were randomly divided into three
groups (n=8 each): group A (control), group B (+10 Gz), group C (+Gz with TP). Group B and C
were repeatedly exposed to +10 Gz (each for 30 s, onset rate about 0.5 G/s, 3 times/d with +1
Gz 1 min intervals, 3 d/wk, 4 weeks in total), but group A was only submitted to +1 Gz. TP(200
mg.kg -1 ) was given orally to group C about 1h prior to the +Gz experiment, and distilled
water was given to group A and B.Function of isolated rat working hearts and myocardial
ultrastructure were observed. Result A significant decrease of left ventricular systolic pressure
(LVSP) and injury of myocardial structure in rats were demonstrated after repeated +10 Gz
stress. But TP could remarkably elevate the LVSP and improve myocardial ultrastructural injury
in +10Gz stressed rats. Conclusion These results indicated that repeated high G exposure may
produce cardiac structural and functional injuries in rats which might be partly related to free
radical metabolism; and antioxidant TP had significant protective effects on the hearts of +Gz
stressed rats.
出处
《航天医学与医学工程》
CAS
CSCD
1999年第2期79-83,共5页
Space Medicine & Medical Engineering
关键词
加速度应激(生理)
心功能
心肌
茶多酚
超微结构
大鼠
acceleration stress(physiology)
heart function
myocardium
tea
polyphenols
ultrastructures
rats
