摘要
采用ＳＯＤ抑制氮兰四唑（ＮＢＴ）在荧光下的还原作用来测定蜂花粉中延衰因子ＳＯＤ活性，以抑制ＮＢＴ光化还原反应的５０％为一个酶活性单位。测定新鲜茶花花粉、商品茶花花粉、蜂粮和新鲜蚕豆花粉中ＳＯＤ活性（ｕ／ｇ鲜重）分别为１５６．３、１０１．２、１１８和３１．６；对这些样品中的ＳＯＤ同工酶进行分离及活性染色，得到蚕豆花粉的ＳＯＤ同工酶具有４条透明的活性谱带。
The activity of superoxide dismutase(SOD) was assayed with the method of SOD suppressing the reduction of NBT in fluorescence.An enzymatic activity unit is defined as half of SOD suppressing NBT photorecovery.The activity of SOD(u/g Fresh Weight)in fresh tea pollen,commercial tea pollen,bee bread and fresh bean pollen was 156.3,101.2,118 and 31.6 The SOD isozyme in these samples was isolated and intravital stained.There were four transparent active band of SOD isozyme in bean pollen.
出处
《中国养蜂》
1999年第1期7-9,共3页
Apiculture of China
