摘要
以抗利福平为标记,采用浇灌根基部接种的方法测定AR03菌株在烟草植株根际的定殖。结果表明,在灭菌土条件下,AR03经人工引入可以有效的定殖于烟草根际土壤和根部,并在一定时间内可维持相当的数量,在根际土壤和根部的定殖动态趋势是先升后降;它们在根部的定殖力均比根际土壤中强。依据传统形态学、生理生化特征及16s rDNA基因序列,菌株AR03为短小芽孢杆菌(Bacillus pumilus)。
Tagged by the resistance to Rif.（300 μg/mL） and inoculated by watering root,under the sterilized soil condition,the rhizosphere bacteria AR03 strain was proved that it could colonized in root and rhizosphere soils of tobacco by artificial induction.AR03 strain could maintain considerable quantity for a while.The colonized dynamics of AR03 strain increased first,then decreased.Besides these,the colonization was greater on root than in rhizosphere.On the basis of observation of colony and cell morphology,biochemical test and the 16s rDNA sequence analysis,Strain AR03 was Bacillus pumilus.
出处
《中国烟草科学》
CSCD
北大核心
2010年第3期45-48,53,共5页
Chinese Tobacco Science
基金
山东省烟草专卖局项目"烟草主要病虫害生物防治技术体系"
青岛科技支撑计划(09-1-1-90-nsh)
四川省烟草专卖局(200703002)
关键词
定殖
鉴定
短小芽孢杆菌
生物防治
colonization
identification
Bacillus pumilus
biological control
