摘要
目的观察多次盐酸灌注豚鼠食管对呼吸道黏膜内神经生长因子(nerve growth factor,NGF)物质表达的影响。方法将20只豚鼠按随机数字表法分为2组,每组10只。模型组:用盐酸氯胺酮将豚鼠轻度麻醉,将5F胃管插入豚鼠食管至食管中下端,灌注含0.5%胃蛋白酶的盐酸(0.1mol/L,8滴/min,20min/d),连续灌注14d;②对照组:用PBS代替盐酸灌注食管,方法同上。用免疫组织化学方法测定两组豚鼠肺组织NGF的免疫反应变化;用免疫印迹法(Western blotting)检测肺组织NGF的免疫反应变化;Metamoph图像分析系统对结果进行分析。结果免疫组织化学结果显示(染色反应强度用灰度值表示):模型组豚鼠气管和细支气管黏膜内NGF物质阳性反应的平均灰度值明显地低于对照组[(108.46±9.89)vs(135.86±4.01)](P<0.01)。Western blotting显示NGF在模型组豚鼠的肺内平均相对光密度值明显强于对照组[(1.415±0.155)vs(0.550±0.039)](P<0.01)。结论多次盐酸灌注豚鼠食管后呼吸道黏膜内NGF物质表达明显增多,提示NGF可能参与胃食管反流性呼吸系统疾病的发病过程。
Objective To observe the expression of nerve growth factor （NGF） in respiratory tract mucosa of the repetitive esophageal stimulation by hydrochloric acid （HCl） in guinea pigs. Methods Twenty adult guinea pigs were randomly divided into two groups（ n = 10）：①HCl model group.. On the day of experimentation, guinea pigs were maintained under ketamine anesthesia. A 5F catheter was inserted through the mouth and into the lumen of the middle and lower oesophagus. The oesophagus of each animal was perfused with HCl P for 20 min/d for 14 d. ②PBS control group：The oesophagus of each animal was perfused with PBS. The alterations of NGF immunoreactivity were investigated by immunohistochemical and Western blotting method and analyzed by the Metamoph analysisi system. Results Immunohistochemistry demonstrated that the mean grey values of NGF positive products decreased significantly in the tracheal epithelia and the distal airway walls of the model guinea pigs compared with the normal PBS control group[（108.46±_9.89） vs （135.86±4.01）]（ P 〈0.01）. Western blotting also demonstrated that NGF expressions were increased in the lungs of the model guinea-pigs than those of normal control group[（1. 415±0. 155） vs （0. 550±0. 039）（ P 〈0.01）. Conclusions NGF in the lungs might be involved in the pathogenesis of the repetitive esophageal stimulation by HCl in guinea pigs.
出处
《中华哮喘杂志（电子版）》
CAS
2010年第2期70-72,共3页
Chinese Journal of Asthma(Electronic Version)
基金
辽宁省高等学校科研资助项目(2004D188)
关键词
胃食管反流
支气管高反应性
神经生长因子
豚鼠
Gastro-esophageal reflux
Bronchial hyperreactiveity
Nerve growth factor
Guinea pig
