摘要
目的:建立HPLC-ESI/MS分析方法测定人血浆中枸橼酸喷托维林的浓度。方法:采用Inertsil ODS-3C18(150mm×2.1mm,5μm)色谱柱,流动相为0.1mol.L-1醋酸铵溶液(甲酸调pH4.4)-甲醇(35:65),流速0.20mL.min-1,柱温为25℃;内标为盐酸洛拉曲克;质谱用电喷雾电离源正离子模式(ESI+),选择离子监测(SIR);样品用固相萃取法处理。结果:枸橼酸喷托维林的线性范围为0.78～300ng.mL-1,r=0.998,喷托维林提取回收率>70%,相对回收率在85%～115%范围内,日内、日间RSD均<15%。结论:该方法专属性强,灵敏度高,结果准确,可用于枸橼酸喷托维林血药浓度测定。
Objective：To establish an HPLC-ESI/MS method for the determination of pentoxyverine citrate in human plasma.Methods：The separation of the analytes was performed on Inertsil ODS-3 C18（150 mm×2.1 mm,5 μm） column using a mobile phase consisting of 0.1 mol·L-1 ammonium acetate solution（pH 4.4,adjusted by formic acid） and methanol （35：65） with a flow rate of 0.20 mL·min-1 at column temperature of 25 ℃.Nolatrexed dihydrochloride was used as internal standard.The compounds were detected in the selected ion recording（SIR）with electrospray ionization source in positive ion（ESI＋）.The samples were extracted using solid phase extraction column.Results：The linear range of pentoxyverine citrate was 0.78-300 ng·mL-1 with r=0.998.The extracted recovery rate of pentoxyverine was larger than 70%,and the relative recovery rates were 85%-115%;The intra-and inter-day RSDs were less than 15%.Conclusion：This method is specific,sensitive and accurate for the determination of pentoxyverine citrate concentration in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第5期853-857,共5页
Chinese Journal of Pharmaceutical Analysis
基金
教育部新世纪优秀人才计划项目(NCET-06-0753)
霍英东高等院校青年教师基金项目(111045)
