摘要
目的通过体外实验，评价人工瓣膜材料细菌粘附与细菌生长的关系。方法采用平板菌落计数法、１２５Ⅰ标记细菌放射性测定法测定金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌和绿脓杆菌的生长曲线，同时测定４种细菌对人工瓣膜材料涤纶、热解碳、聚四氟乙烯的粘附情况。结果４种细菌对３种人工瓣膜材料粘附能力与细菌生长曲线基本一致。细菌在体外生长不受人工瓣膜材料存在的影响，不同时间同一细菌对同一材料粘附不同。结论细菌对材料粘附与细菌生长曲线基本一致。
Objective The bacterial adhesive prosthetic valve materials was studied by investigations in vitro. The method to evaluating bacteria adhesive degree to prosthetic valve materials was established primarily. Methods The adhesive capacities of staphylococcus aureus(SA),staphylococcus epidermidis(SE),Escherichia coli (EC) and Pseudomonas aeruginosa(PA) to Dacron, pyrolytic carbon and polytetrafluoroethylene(PTFE) were quantitatively determined respectively by the plate counting and gamma ray counting of 125 Ⅰ radiolabeled bacteria in vitro. Results The adhesive capacities of four types of bacteria to Dacron, pyrolytic carbon and PTFE were coincidence with the bacterial growth curve. The adhesive capacities of four bacteria to Dacron were stronger and the adhesion of SE to pyrolytic carbon was the strongest. The adhesion of PA remained to a high level. The adhesive capacities of EC and PA to PTFE were the strongest. Conclusion The adhesive capacities of one bacterium to different prosthetic valve materials and different bacteria to one prosthetic valve materials were different.
出处
《中国胸心血管外科临床杂志》
CAS
1999年第1期7-9,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
国家自然科学基金
关键词
人工瓣膜
生物材料
细菌生长
细菌粘附
Prosthetic valve Biomaterials Bacterial growth Bacterial adhesion
