摘要
目的评估发光二极管荧光显微镜实验室诊断结核分枝杆菌的能力。方法收集409痰标本,以固体罗氏培养为金标准,比较发光二极管荧光显微镜与普通光学显微镜、普通荧光显微镜在敏感性、特异性及检出时间上的差异,并比较技术人员对几种方法的评价。结果罗氏培养阳性的175份痰标本中,普通光学显微镜法112份为阳性,普通荧光显微镜法121份为阳性,发光二极管荧光显微镜法130份为阳性,敏感性分别为64.0%,69.1%,74.3%;罗氏培养阴性的234份痰标本中,普通光学显微镜法233份为阴性,荧光显微镜法227份为阴性,发光二极管荧光显微镜法227份为阴性,特异性分别为99.6%,97.0%,97.0%。χ2检验统计结果表明,发光二极管荧光显微镜与普通光学显微镜、普通荧光显微镜检测结果差异有统计学意义(P<0.001)。普通光学显微镜、普通荧光显微镜和发光二极管荧光显微镜读片所用的读阳性片时间分别为186.7 s、75.1 s、72.2 s,发光二极管荧光显微镜与普通光学显微镜在读阳性片时间上差异有统计学意义(P<0.05),与普通荧光显微镜差异无统计学意义。参与研究的技术员评价发光二极管荧光显微镜较其他2种方法易接受。结论发光二极管荧光显微镜较普通光学显微镜及普通荧光显微镜在检查效果及检出时间上存在明显优势,对技术人员的亲和性好,具有推广使用的价值。
Objective To assess the performance of fluorescence microscopy with light emitting diodes（LEDFM） in detecting Mycobacterium tuberculosis. Methods Using solid L-J culture（L-J） as gold standard,409 sputum samples were detected by Ziehl-Neelsen（ZN）,conventional fluorescence microscopy（FM） and LEDFM.Their sensitivities,specificities,detection times,and feeling of the user were compared. Results Of 175 L-J-positive samples,112 samples were ZN positive,121 samples were FM positive,130 samples were LEDFM positive.Their sensitivities were 64.0%,69.1%,74.3%,respectively.Of 234 L-J-negative samples,233 samples were ZN negative,227 samples were FM negative,227 samples were LEDFM negative.Their specificities were 99.6%,97.0%,97.0%,respectively.There were significant difference among the results of LEDFM and the other two methods（P0.001）.Detection times of ZN-,FM-,LEDFM-positive slides were 186.7 sec,75.1 sec,72.2 sec,respectively,in which there was significant difference between ZN and LEDFM（P0.05）,but not between FM and LEDFM.LEDFM was highly appreciated by technicians involved in this study. Conclusions LEDFM had better efficacy and use value in detecting M tuberculosis than ZN and FM.
出处
《中国防痨杂志》
CAS
2010年第5期275-279,共5页
Chinese Journal of Antituberculosis
关键词
分枝杆菌
结核
显微镜检查
荧光
Mycobacterium tuberculosis
microscopy
fluorescence
