摘要
2008和2009年在对木薯主产区病害的普查中,发现了一种新的叶斑病,命名为离孺孢叶斑病。形态观察结果表明,该病原菌分生孢子梗丛生,褐色,基细胞膨大呈半球形,成熟的分生孢子长椭圆形,两端钝圆,具5～8个隔膜,表明其为狗尾草离孺孢。ITS序列分析结果表明其和一株狗尾草离孺孢(EF452444)的序列同源性为99%。生物学特性研究结果表明,PDA培养基、28℃、pH7和光暗交替是菌落生长最适条件,D-葡萄糖和硝酸钠是其最适碳氮源,孢子萌发最适温度和致死温度分别为28℃和55℃5min。10种杀菌剂的室内筛选结果表明,咪鲜胺(25%咪鲜胺EC)的EC50值最小,抑菌效果最好。
A new leaf spot disease was first found during a survey made from 2008 and 2009 in cassava main growing regions. Microscopic examination showed the conidiophores were fasciculate and brown, and that the basal cell was enlarged and hemispheric. Well-developed conidia were long-obclavate, obtuse at both ends, with five to eight transverse septa, which indicated that the pathogen was Bipolaris setaria. The ITS sequence of the pathogen had homology of 99% with a sequence of B. setaria（EF452444）. The optimum condition for mycelial growth included PDA medium, 28 ℃, pH7 and alternative light, and optimum carbon and nitrogen sources were D-glucose and sodium nitrate, respectively. The optimum temperature for conidia germination was 28 ℃ and the lethal temperature for conidia was 55 ℃ for 5 min. Ten fungicides were screened in laboratory, and prochloraz 25% EC was found to have the lowest EC50 value and best inhibition activity against the pathogen.
出处
《热带作物学报》
CSCD
2010年第3期457-463,共7页
Chinese Journal of Tropical Crops
基金
国家木薯现代产业技术体系建设项目(No.nycytx-17-37)
农业部公益行业科研专项(No.nyhyzx07-13-5)
关键词
木薯
离蠕孢
生物学特性
杀菌剂
cassava
Bipolaris setariae
biological characteristics
fungicides.
