摘要
目的：探讨脑活素对新生大鼠缺氧缺血性脑病（ＨＩＥ）防治作用的机理。方法：选用７日龄Ｗｉｓｔａｒ大鼠，在制成ＨＩＥ模型前后给予腹腔注射脑活素各一次，在ＨＩＥ后４８ｈ处死测定脑组织一氧化氮（ＮＯ）含量、一氧化氮合酶（ＮＯＳ）活性、ＮＯＳ免疫组化及细胞凋亡，并与正常对照组及ＨＩＥ组的测定结果比较。结果：ＨＩＥ组ＮＯ含量、ＮＯＳ活性及凋亡细胞比例明显高于正常对照组的测定值，ＮＯＳ阳性神经元的阳性强度及数量也较正常对照组高。而脑活素干预组的测定值均明显低于ＨＩＥ组的测定值。结论：脑活素对ＨＩＥ具有防治作用，其机理与阻断ＮＯ异常代谢、降低细胞凋亡有关。
Objective: The purpose of this study is to research the prevention and treatment effect and mechanism of cerebrolysin on hypoxic ischemic encephalopathy(HIE).Methods: Using 7 day newborn Wistar rats,cerebrolysin was injected intraperitoneally before and after hypoxia and ischemia with the dosage of 12.76 mg/20 g.Rats were killed after HIE according to the different schedules.The parameters of nitric oxide、nitric oxide synthase、nitric oxide synthase protein expression and apoptosis were measured,which were compared with that of normal control group and HIE group.Results: The containt of NO、the activity of NOS and the ratio of apoptosis were much higher in the HIE group than that of normal control group.The number and the positive intensity of NOS positive neuron was also much higher in HIE group.The values in the cerebrolysin group were lower than that of HIE group.Conclusion: Cerebrolysin has the protection and treatment effect on HIE.The mechanism of the effect is correlated with blocking abnormal metabolism of NO and reducing apoptosis of neuron.
出处
《河南医学研究》
CAS
1999年第1期15-16,共2页
Henan Medical Research
基金
河南省医学科学院科研基金
关键词
脑活素
缺氧缺血性脑病
一氧化氮
新生儿
防治
cerebrolysin
hypoxic ischemic encephalopathy
nitric oxide
nitric oxide synthase
