摘要
目的比较山豆根全组分、水提组分、醇提组分、总生物碱提取物对小鼠的急性毒性,为山豆根的临床安全使用提供实验依据。方法采用经典的急性毒性实验方法测定山豆根全组分的最大耐受量(MTD)及水提组分、醇提组分、总生物碱提取物的小鼠口服半数致死量(LD50),观察其急性毒性症状谱,记录累积死亡数及小鼠体重变化。结果山豆根全组分的MTD为10.68g·kg-1,水提组分、醇提组分、总生物碱提取物的小鼠口服LD50及95%可信限分别为17.469(15.450～19.701)g·kg-1、27.135(24.869～29.622)g·kg-1、13.399(12.016～14.899)g·kg-1,主要毒性症状是烦躁、多动、呼吸急促、抽搐。结论山豆根不同组分急性毒性大小顺序依次是总生物碱提取物、水提组分、全组分、醇提组分,其毒性成分与致毒机制尚待进一步研究。
Objective Compare the acute toxicity of different components of Radix et Rhizome Sophorae Tonkinensis and to provide experimental data for safe use in clinal.Methods Different components of Radix et Rhizome Sophorae Tonkinensis were administrated to KM mice.The MTD of all-components and the LD50 of water extract,alcohol extract and total alkaloid extract of Radix et Rhizome Sophorae Tonkinensis were tested by the classical methods of acute toxicity.The acute toxic symptoms were observed;the accumulated death number and the weight-changes of live mice were recorded.Results The MTD of all-components of Radix et Rhizome Sophorae Tonkinensis was 10.68g·kg-1.The LD50 of water extract,alcohol extract and total alkaloid extract of Radix et Rhizome Sophorae Tonkinensis were 17.469（15.450～19.701）g·kg-1,27.135（24.869～29.622）g·kg-1,13.399（12.016～14.899）g·kg-1 in mice.Restlessness,breathlessness and convulsion were the main acute tixical symptoms.Conclusion The size order of acute toxicity of different components in Radix et Rhizome Sophorae Tonkinensis is total alkaloid extractwater extract all-componentsalcohol extract.The toxical components and toxical mechanism should be researched furtherly.
出处
《中国药物警戒》
2010年第5期257-262,共6页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题
(课题编号:2008GG2NS02021)
关键词
山豆根
全组分
水提组分
醇提组分
总生物碱提取物
急性毒性
Radix et Rhizome Sophorae Tonkinensise
all-components
water extract
alcohol extract
total alkaloid extract
acute toxicity
