摘要
目的观察苏木水提物对人类浆液性囊腺卵巢癌细胞SKOV3的生长抑制及诱导凋亡作用。方法采用细胞计数法绘制生长抑制曲线；光镜下观察药物作用后SKOV3细胞经锥虫蓝染色后的形态变化；电子显微镜下观察苏木水提物作用后细胞的超微结构；不同质量浓度苏木水提物作用于细胞后，用流式细胞术（FCM）进行凋亡及周期分析。结果生长曲线显示苏木水提物对SKOV3细胞有明显的生长抑制作用，且呈现一定的质量浓度依赖性；电镜图可见典型的细胞凋亡特征，如细胞核固缩、染色质凝聚及凋亡小体形成；FCM分析证实：体外培养的SKOV3细胞经不同质量浓度的苏木水提物作用后，细胞生长明显受到抑制，细胞凋亡率随苏木水提物质量浓度的增加而增加。结论苏木水提物对人类卵巢癌细胞株SKOV3有明显的生长抑制及诱导凋亡作用。
Objective To observe caesalpinia sappan aqueous extract＇s growth inhibiting and apoptosis inducing effects on human ovarian cancer cell line SKOV3. Methods Cell growth inhibiting effect was detected by cytometry; Examined were by trypan blue staining under a light microscope; Electronic microscopy was used to observe uhrastructure of SKOV3 cell affected by caesalpinia sappan aqueous extract＇s; With different concentration of eaesalpinia sappan aqueous extraet＇s acting on cells, cells cycle and apoptosis rate were analyzed by flow cytometry （FCM）. Results The growth of SKOV3 was inhibited effectively by caesalpinia sappan aqueous extract＇s; typical morphology of apoptosis （cell shrinkage, chromatin condensation and apoptotic body formation） were observed and in a concentration-dependent manner. Flow eytometry detection showed： the experiment in vitro revealed growth of SKOV3 after treatment by eaesalpinia sappan aqueous extract ~s at the different concentration. Apoptosis rate was increased with the increasing of the concentration of eaesalpinia sappan aqueous extract＇s. Conclusion Cacsalpinia sappan aqueous extract＇s can obviously inhibit growth of human ovarian cancer cell line SKOV3 and induce its apoptosis.
出处
《肿瘤研究与临床》
CAS
2010年第4期243-246,共4页
Cancer Research and Clinic
基金
基金项目：山西省实验动物专项资金项卧2007（k01）]
关键词
肿瘤
实验性
卵巢肿瘤
苏木水提物
细胞凋亡
Neoplasms, experimental
Ovarian neoplasms
Caesalpinia sappan aqueous extract＇s
Apoptosis
