摘要
将28只日本大耳兔随机分为实验组(20只)和对照组(8只),实验组用旋毛虫脱囊幼虫经口灌胃日本大耳兔(3000条/只),对照组不做任何处理。采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清。建立旋毛虫肌肉幼虫排泄分泌抗原(MLESA)为诊断抗原的间接ELISA,测定兔唾液和血清中抗旋毛虫IgG抗体。结果显示,感染后1～6周,唾液阳性率分别为10%、15%、40%、65%、85%和95%;血清阳性率分别为35%、50%、80%、90%、100%和100%。感染后1～3周,唾液阳性率与血清阳性率差异有统计学意义(χ2=3.58、5.23、6.67,P<0.05),感染后4～6周,两者差异无统计学差异(χ2=0.12、1.03、1.03,P>0.05)。提示在血清标本采集困难的情况下,MLESA的间接ELISA法检测唾液中抗旋毛虫IgG抗体可作为旋毛虫病免疫诊断的辅助方法。
Twenty-eight Japanese big ear rabbits were randomly divided into control group and experimental group. Twenty rabbits in experimental group were each infected with 3 000 larvae of Trichinella spiralis. Serum and saliva samples were collected at pre-infection and every week after infection, and were examined for IgG antibody by indirect ELISA using T. spiralis muscle larvae excretory-secretory antigen （MLESA）. At 1, 2, 3, 4, 5 and 6 weeks afer infection, the positive rate in saliva samples was 10%, 15%, 40%, 65%, 85%, and 95%, respectively; and that of serum samples was 35%, 50%, 80%, 90%, 100%, and 100%, respectively. The positive rate was significantly different between saliva and serum samples at 1, 2 and 3 weeks post-infection （χ2=3.58, 5.23, 6.67, P0.05）, but no significant difference at 4, 5, and 6 weeks post-infection （χ2=0.12, 1.03, 1.03, P0.05） . The results indicate that the indirect ELISA using MLESA to detect IgG antibody in saliva may be helpful for clinical diagnosis of trichinellosis.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2010年第2期112-114,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
旋毛虫
肌肉幼虫
排泄分泌抗原
IGG
唾液
血清
Trichinella spiralis
Muscle larvae
Excretory-secretory antigen
IgG
Saliva
Serum
