摘要
利用微波技术研究灵芝三萜类化合物的提取工艺条件。以乙醇浓度、提取时间、功率、液料比、温度为工艺参数,通过单因素及Box-Benhnken模型,做四因素三水平响应面优化提取条件试验;通过响应面模型的回归分析,获得最佳提取工艺条件:乙醇体积分数75%、提取温度75℃、功率870W、液料比33 mL/g、时间17 min。在此条件下灵芝三萜的平均提取率为1.043%,一次提取率可达93%。与未经微波处理的超声法、回流法、浸提法相比,微波处理可使三萜提取率提高150%。超声法、回流法和浸提法的三萜提取率分别为微波提取法的72%、86%和63%,且提取时间长,溶剂耗量大。采用微波技术提取灵芝三萜类的工艺,稳定性好,提取率高。
The optimum extraction conditions of microwave technology were studied for the triterpenoid saponins from Ganaderma lucidum.The extraction parameters as ethanol concentration,extraction time,extraction power,the ratio of water to sample and the temperature of extraction were optimized based on single-factor experiment and three-level fourfactor box-behnken design.According to response surface software regression analysis,the result showed that the optimum conditions were as follows,the concertration ethanol 75%,extraction temperature 75 ℃,extraction power 870 W,ratio of water to sample 33 mL/g,extraction time 17min.The average yield rate of triterpenoid saponins from Ganoderma lucidum was 1.043% with high stability,and one-time extraction was up to 93%.Compared with non-microwave extraction,the triterpenoid saponins exaction rate was up 150% by microwave technology.The ultrasound extraction rate was only 72% of microwave exaction rate under same condition.The heat reflux extraction rate and lixiviation were 86% and 63% of microwave exaction rate respectively.It was concluded that microwave extraction had several remarkable advantages as the shorter extraction time,the less solvent,the higher extraction rate.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2010年第2期89-96,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省科技厅项目(No.2008C32030)
关键词
微波提取
灵芝三萜
响应面法
microwave extraction
triterpenoid saponins
Response Surface Methodology
