摘要
目的检测大肠癌组织中PTEN和Caspase-3的蛋白表达,了解与大肠癌临床病理的相关因素。方法采用S-P免疫组化法,检测PTEN和Caspase-3在各组织中的表达及意义。结果PTEN在正常大肠组织、大肠腺瘤、大肠癌组织中的表达率分别为100%、70%和51.61%;而癌旁组织的阳性表达率60.87%,与癌组织相似,两者有明显的同步表达性(P<0.01)。大肠癌中PTEN蛋白的阳性表达率明显低于非癌组(P<0.05)。Caspase-3在正常黏膜、大肠腺瘤及大肠癌中的阳性表达率分别为93.33%、60%、45.16%,在癌旁组织的表达率为52.17%。正常黏膜组织Caspase-3的表达高于大肠腺瘤和大肠癌(P<0.05)。PTEN表达随肿瘤分化程度的降低、临床分期提高而降低(P<0.05),Caspase-3阳性表达随肿瘤分化程度的降低而降低(P<0.05)。大肠癌组织中PTEN与Caspase-3的表达呈正相关(r=0.675,P<0.01)。结论PTEN、Caspase-3的表达下调与大肠癌的发生、发展有关,二者可作为判定大肠癌生物学行为和预后的临床参考指标。
Objective To determine the expression of PTEN and Caspase-3 in colorectal carcinoma and investigate the relationship between them and the clinicopathologic features.Methods The S-P immunohistochemical method was used to detect the expression and significance of PTEN and Caspase-3 in colorectal tissues.Results Expressions of PTEN in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers are 100%、70% and 51.61%,respectively.Expression of PTEN in paracarcinoma mucosa is 60.87%,similar to that in colorectal cancer.Expression of PTEN in colorectal cancer is lower than that in non-cancer groups（P〈0.05）.Expressions of Caspase-3 in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers are 93.33%、60%、45.16%,respectively.And expression in paracarcinoma mucosa is 52.17%.Expression of Caspase-3 in normal colorectal mucosa tissue is higher than that in colorectal adenoma tissue and colorectal cancer（P〈0.05）.The expressions of PTEN and Caspase-3 in well-differentiated adenocarcinoma were higher than that in poorly-differentiated adenocarcinoma（P〈0.05）.Also,the lost or decreased expression of PTEN significantly correlated with clinicalpathological stage .Significant relationships were found between PTEN and Caspase-3（r =0.675,P〈0.01）.Conclusion The experiment suggests that PTEN and Caspase-3 may play certain roles in the oncogenesis and progression of colorectal cancer.Their expressions may be favorable markers and useful prognostic indicators in the patients with colorectal cancer.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第4期290-292,共3页
Journal of China Medical University
基金
辽宁省博士启动基金资助项目(20081048)
