摘要
目的探讨缺血后处理(I-postC)对大鼠双侧后肢缺血再灌注(I/R)后肺损伤的影响及意义。方法阻断肾下腹主动脉建立大鼠双侧后肢I/R损伤模型。48只大鼠随机分为3组:缺血再灌注(I/R)组、缺血预处理(IPC)组及I-postC组,每组再随机分为两个亚组(n=8),并分别于再灌注后12h,24h取标本。观察肺组织形态学、湿/干重比、丙二醛(MDA)及髓过氧化物酶(MPO)的变化。原位杂交和逆转录多聚酶链反应(RT-PCR)方法检测肺组织中细胞间黏附分子1(ICAM-1)的mRNA表达,Western blot检测ICAM-1蛋白表达。结果IPC组和I-postC组的各项指标均明显减少,与I/R组比较差异十分显著(P<0.01),但两组之间差异不显著(P>0.05)。结论I-postC能减轻大鼠双侧后肢缺血再灌注后肺损伤,与IPC可能存在共同的作用机制。
Objective To study the effect of ischemic postconditioning（I-postC）on the lung injury following ischemia-reperfusion（I/R）in the hind limbs of rats.Methods The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours.48 rats were divided into 3 groups：I/R group,IPC and I-postC group.Each group received 4 hours of ischemia and then 12 hours or 24 hours of reperfusion respectively.The tissue morphology,wet-to-dry weight（W/D）ratio,malondialdehyde（MDA）and myeloperoxidase（MPO）in lung were compared.The mRNA expression of ICAM-1 in lung was also studied by RT-PCR or in situ hybridization.The protein product was detected by Western blot.Results In IPC and I-postC group,all parameters decreased significantly compared with I/R ischemia group（P〈 0.01）.There was no significantly difference between the IPC and I-postC group（P 〈0.05）.Conclusion I-postC can attenuate lung injury following skeletal muscle I/R in the hind limbs of rats,which maybe accomplished by inhibition of adhesion and infiltration of polymorphonuclear neutrophil（PMN）.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第4期245-247,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30600631
30672048)
关键词
缺血后处理
缺血再灌注
骨骼肌
肺损伤
ischemic postconditioning
ischemia-reperfusion
skeletal muscle
lung injury
