摘要
以红三叶 (Trifolium pratense)野生品种巴东红三叶的叶片为外植体 ,在MB - 1至MB - 9的固体培养基上诱导愈僵组织 ,筛选出最佳培养基是MB - 9培养基 以白三叶(Trifoliumclover)栽培品种“路易斯安娜”和“Huca”的叶片为外植体 ,接种在MB - 9固体培养基上诱导愈伤组织 愈僵组织形成以后 ,均经MB - 9固体培养基上继代培养 3～ 4次后 ,获得胚性愈伤组织并建立胚性细胞悬浮系 实验结果表明 ,缩短换液时间 ,即每隔 3～ 4gd换一次 。
Usung leaves of wild Batong Trifolium Pratens as explant,calli were induced on from MB-1 to MB-9 solid medium.After screening,the best medium was MB-9,Using leaves of cultivated Trifolium Repens,namely “,as expant,them were put on the MB-9 solid medium.After calli was formed,were subcultured on the same solid medium for 3-4 times and embrogemic calli were obtained,then embrogenic calli were tranferred into MB liquild medium with 3mg/L2,4-D,0.7mg/L BA,0.2mg/L NAA,2mg/L GL y, 500mg/L CH,3%sucrose.After strengthened cultruing for 2 months,the embrogenic cell suspension was obtained.The test showed that shortening the time of exchanging liquid,namly once 3-4d,conld quicken establishing embrogenic cell suspension.”
出处
《松辽学刊（自然科学版）》
1998年第3期32-35,共4页
Songliao Journal (Natural Science Edition)
关键词
红三叶
白三叶
愈伤组织
胚性细胞悬浮系
Badong Trifolium Pratense Trifolium repens Calli embrogenic cell suspension
