摘要
目的了解兔颈部胸骨舌骨肌的血供解剖特点,并尝试在该肌肉内构建组织工程管状软骨。方法用红色乳胶灌注新西兰兔的动脉系统,显示营养胸骨舌骨肌的动脉血管分布情况。将兔自体软骨细胞-PGA/PLA复合物在体外培养2周后,植入兔胸骨舌骨肌内继续培养4周,取材进行相应的组织学检测,并与正常兔气管组织进行比较。结果兔胸骨舌骨肌由颈总动脉的2个直接分支供血。其中第一分支的发出点接近胸骨舌骨肌中点,供应整块肌肉;第二分支发出点偏舌骨端,主要供应肌肉上半部分。体内培养4周后,在兔胸骨舌骨肌内可见规则的管状软骨组织,表面形成致密的血管网络。HE染色见软骨细胞排列规则,包埋在软骨陷窝内,表层细胞较大、深部细胞较小;SafraninO染色呈强阳性,表示有丰富的蛋白聚糖基质;Ⅱ型胶原免疫组化显示,新生软骨陷窝周围呈强阳性反应,表明有大量Ⅱ型胶原分泌。新生软骨的特性与正常气管软骨类似。结论在兔胸骨舌骨肌内可以构建出满意的组织工程管状软骨。本研究为应用胸骨舌骨肌预构肌肉-软骨复合组织瓣修复气管缺损进行了探索。
Objective To explore the vascular anatomy of rabbit sternohyoid muscle and construct tubular cartilage inside the muscle. Methods Red latex perfusion was used to analyze the vascular pattern of rabbit sternohyoid muscle. Autologous chondrocyte-PLA/PGA constructs were pre-cuhured for 2 weeks in vitro, wrapped by sternohyoid muscle for another 4 weeks, and then harvested for gross observation and histological analysis. Normal rabbit tracheal cartilage served as control. Results Rabbit sternohyoid muscle was directly supplied by two branches from common carotid artery. One branch entered the muscle in the middle and supplied the whole muscle, while the other entered beside the hyoid end and supplied the upper half. After 4 weeks of culture in muscle pocket, the cell-scaffold construct developed into a cartilage tube with dense anastomosis on its surface. HE staining showed that the chondrocytes were arranged regularly in lacuna,with large cells on the surface and small cells in the deep. Safranin 0 staining showed strong positive results, indicating abundant proteoglycan in the matrix. Immunochemistry showed strong positive results around the lacune, suggesting massive type II collagen secretion. All indicated that the neo-cartilage had similar properties to normal rabbit tracheal cartilage. Conclusion The pocket of sternohyoid muscle is an ideal site for cartilage engineering in vivo. The results may act as an experimental basis for further prefabrication of muscle-cartilage composite in the repair of tracheal defect.
出处
《组织工程与重建外科杂志》
2010年第2期78-81,共4页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
组织工程
软骨
胸骨舌骨肌
Tissue engineering
Cartilage
Sternohyoid muscle
