摘要
目的制备、纯化抗人骨肉瘤OS-732单克隆抗体。方法通过杂交瘤细胞腹腔注射BALB/c小鼠制备腹水,ProteinA-Sepharose CL-4B亲和层析法纯化单抗,SDS-PAGE、免疫组化及ELISA检测该单抗的性质。结果SDS-PAGE鉴定单抗纯度达到93%,ELISA检测抗体免疫活性效价为1×10-7。结论制备、纯化了具有较高纯度及生物活性的的抗骨肉瘤OS-732单克隆抗体,为下一步骨肉瘤靶向治疗打下了基础。
Objective To prepare and purify the murine antibody against human osteosarcoma OS-732.Methods Human osteosarcoma OS-732 and hybridoma cells were injected into the abdominal cavity of BALB/c mice to produce monoclonal antibody against human osteosarcoma.The antibody was purified by protein A-sepharose CL-4B affinity chromatography and its properties were evaluated by SDS-PAGE,immunohistochemistry and ELISA.Results SDS-PAGE showed that the purity of monoclonal antibody was about 93%.ELISA revealed that the immunologic activity of monoclonal antibody was 1×10-7.Conclusion Monoclonal antibodies with a high purity and immunological activity can be employed in further study of targeted therapy for human osteosarcoma.
出处
《军医进修学院学报》
CAS
2010年第5期462-464,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家"863"计划项目(2007AA021806)~~
关键词
骨肉瘤
杂交瘤
抗体
单克隆
亲和层析
Osteosarcoma
Hybridoma
Antibodies
Monoclonal
Affinity Chromatography
