摘要
在已经建立的支气管败血波氏杆菌(Bb)、多杀性巴氏杆菌(Pm)、副猪嗜血杆菌(HPS)的单项PCR诊断方法的基础上,通过对扩增条件的优化,成功建立在一个循环条件下同时检测3种细菌的PCR实验室诊断方法。利用一次PCR即可同时扩增出Bb的237bp、Pm的457bp,和HPS的821bp的特异性片段。该方法可检测到1.34×103cfu1/mLBb,1.6×102cfu/mLHPS,2.6×102cfu/mLPm。该方法的敏感性与已报道的各单项PCR方法的敏感性一致。该方法的建立对临床上进行这3种疾病的鉴别诊断和混合感染的检测都具有重要意义。
A PCR detecting Bb, Pm and HPS was developed based on published single PCRs for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized. A 237 bp fragment of Bb, a 457 bp of Pm and a 821 bp of HPS can be simultaneously amplified in one condition .The PCR can detect minimum 1.34×103cful/mL cells of Bb,2.6×102cfu/mL cells of Pm, 1.6×102cfu/mL cells of HPS. The sensitivity of the PCR is quite comparable with their reported uniplex PCRs.The assay is a potential tool for differentiation of the 3 pathogens and diagnosis of mixed infection by these bacteria.
出处
《畜禽业》
2010年第5期57-59,共3页
Livestock and Poultry Industry
