摘要
目的观察肝癌缺失基因1(DLC-1)对HT-29细胞侵袭能力的影响。方法用脂质体法将重组质粒pcDNA3.1-DLC-1转染HT-29细胞;应用Rho激酶(ROCK)特异性抑制剂Y27632处理HT-29细胞;Westernblot检测p-MLC蛋白表达;Transwell小室实验检测细胞侵袭能力。结果野生型HT-29细胞DLC-1基因表达呈阴性,经转染获得DLC-1稳定表达细胞系;与对照组及空载组HT-29细胞相比,转染组和ROCK抑制剂组p-MLC蛋白表达均下调,细胞侵袭能力受到抑制(P<0.05)。结论转染DLC-1基因可能通过抑制ROCK活性,从而抑制HT-29细胞的侵袭能力。
Objective To investigate the effects of frequently deleted in liver （DLC-1） gene transfection on HT29 cell invasion.Methods Recombinant pcDNA3.1 vector containing the DLC-1 gene was constructed and transfected into HT29 cell line.HT29 cell was treated with Rho-associated kinase（ROCK） inhibitor Y27632.The expression of p-MLC was examined by Western blotting.The invasion ability was detected by transwell.Results The HT-29 cells rarely expressed DLC-1 mRNA and the transfected HT-29 cells could express DLC-1 mRNA steadily.Significant down-regulation of p-MLC expression and decreased invasive ability of DLC-1 were seen in groups of transfected and ROCK inhibited HT29 cells.Conclusion Overexpression of DLC-1 gene caused significantly down-regulation of ROCK activity,which may further inhibit the invasiof HT29 cells.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第6期685-687,F0002,共4页
Jiangsu Medical Journal
基金
国家科学自然基金(30471937)
高等学校博士学科专项科研基金(200802860040)
