摘要
目的:初步探索组织工程室在兔体内脂肪组织工程中的应用;建立研究兔体内脂肪组织自发生长的动物模型。方法:制作中空的表面带孔的硅胶组织工程室;将兔体内以腹壁下浅动脉为蒂的脂肪瓣转移至中空的硅胶组织工程室后6周取材。结果:硅胶组织工程室能很好的支持室内脂肪瓣的生长;所有组织工程室内内容物体积增大,脂肪瓣外面形成一层结缔组织构成的包膜,包膜穿过组织工程室表面的孔与室外包膜交通。室内内容物体积从(0.5225±0.031)ml增加到(3.7625±0.106)ml,包膜体积大小为(0.7525±0.04)ml,脂肪瓣的体积从(0.5225±0.031)ml增加到(3.01±0.135)ml,增加了476%。结论:硅胶制作的组织工程室能应用到体内脂肪组织工程,且为室内组织提供空间生长;成功构建了兔体内组织工程室内脂肪组织自发生长的模型,带蒂的脂肪组织可以在不添加任何细胞,生长因子的情况下自发生长。
Objective To explore the application of tissue engineering chamber in in vivo adipose tissue engineering,to establish a model of spontaneous generation of new adipose tissue from an existing fat flap.Methods A defined volume of fat flap based on the superficial inferior epigastric vascular pedicle was elevated and inset into a hollow silicon chamber implanted subcutaneously in the groin of the rabbit.The contents were analyzed after being in situ for 6 weeks.Results The total volume of construct in all tissue engineering chambers increased significantly,from0.5225±0.031ml baseline compared to（3.7625±0.106）ml.The volume of the fat flap which included the adipocyte and blood vessels volume increased from（0.5225±0.031）ml at insertion to（3.01±0.135）ml（476%increase）in the perforated silicon chambers.Histomorphometric analysis rather than simple hypertrophy documented an increased number of adipocytes.The new tissue was highly vascularized and no fat necrosis or atypical changes were observed.Conclusion A novel method of spontaneous generation of new adipose tissue from an existing fat flap in rabbit is established.Tissue growth is spontaneous and needs no additional cells,growth or differentiation factors,or extracellular matrices.
出处
《中国美容医学》
CAS
2010年第4期518-520,共3页
Chinese Journal of Aesthetic Medicine
关键词
组织工程室
脂肪瓣
脂肪组织工程
兔
tissue engineering chamber
fat flap
adipose tissue engineering
rabbit
