摘要
采用顶空吸附法和气相色谱-质谱法研究了两个Bt玉米品种(5422Bt1,5422CBCL)和一个同源常规玉米品种(5422)在健康和经斜纹夜蛾取食危害后两种状态下释放的挥发物组成和含量差异.结果表明:健康状态下,5422释放了(E)-β-罗勒烯、芳樟醇、(3E)-4,8-二甲基-1,3,7-壬三烯(DMNT)和(E)-β-法尼烯4种挥发物;5422Bt1释放了芳樟醇和DMNT2种挥发物;而5422CBCL只释放芳樟醇1种挥发物.虫害后,在5422和5422Bt1的挥发物中鉴定出了萜类、醇类、酯类和吲哚等12种化合物;在5422CBCL中鉴定出了10种,缺少(Z)-3-己烯-1-醇乙酸酯和橙花叔醇;3个品种释放的萜烯类化合物种类和含量均明显增加,其中,5422和5422Bt1增加了8种,5422CBCL增加了7种;5422Bt1和5422CBCL的芳樟醇和DMNT释放量高于5422,其余化合物的释放量均低于常规品种,尤其是(E)-β-法尼烯显著低于5422.可见,经斜纹叶蛾取食诱导后,Bt玉米释放的挥发物种类和含量与常规玉米不同,且不同的Bt玉米品种(Bt11与MON810)之间也存在差异.
By the methods of headspace enrichment and GC-MS,this paper studied the composition and quantity of the volatiles emitted from two transgenic Bt maize varieties（5422Bt1 and 5422CBCL） and their homologous regular variety（5422） after the feeding by Spodoptera litura.As for the intact plants,the volatiles emitted from variety 5422 were（E）-β-ocimene,linalool,（3E）-4,8-dimethyl-1,3,7-nonatviene（DMNT）,and（E）-β-farnesene,those from 5422Bt1 were linalool and DMNT,while 5422CBCL only emitted linalool.After the feeding by S.litura,a total of twelve volatiles including terpenoid,alcohol,ester,and indole were emitted from 5422 and 5422Bt1,and ten volatiles were emitted from 5422CBCL,with a lack of（Z）-3-hexen-1-yl acetate and nerolidol.The kinds and quantities of terpenoids emitted from the test three varieties were increased significantly,with eight kinds increased for varieties 5422 and 5422Bt1,and seven kinds increased for 5422CBCL.The emitted amounts of linalool and DMNT from the Bt maize varieties were higher than those from the regular variety,while the emitted amounts of other volatiles were in reverse.Especially for（E）-β-farnesene,its emitted amount from the Bt maize varieties was much lower than that from the regular variety.It was obvious that after the feeding by S.litura,the composition and quantity of the volatiles emitted from the Bt maize varieties differed with those emitted from the regular variety,and there was a difference between varieties 5422Bt1 and 5422CBCL.
出处
《应用生态学报》
CAS
CSCD
北大核心
2010年第4期1007-1013,共7页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30470335
30770402)
广东省自然科学基金项目(E039254
06025813)
广东省科技计划项目(2006B50104002
2007A020300009-1)资助
关键词
斜纹夜蛾
BT玉米
挥发物
组成
含量
Spodoptera litura
Bt maize
volatiles
composition
quantity.
