摘要
利用高效液相色谱-DAD-化学发光法(HPLC-DAD-CL)在线检测麦冬对超氧阴离子(O2.-)的清除活性,追踪其抗氧化活性的物质基础。将麦冬水提物分成乙醚和正丁醇两个萃取部位,以化学发光的降低作为检测指标,测定麦冬中主要活性成分对邻苯三酚-鲁米诺-碳酸缓冲液体系产生的O2.-的清除能力,并利用HPLC-DAD-MS/MS和对照品对其进行结构鉴定。采用槲皮素为阳性对照,通过标准效价方程分别评价了主要活性成分的抗氧化活性。研究结果表明,麦冬中直接清除超氧阴离子作用主要来自乙醚部位中的高异黄酮成分。HPLC-DAD-CL在线联用技术简单、快速,可用于中草药抗氧化活性成分的筛选。
The scavenging activities of Ophiopogon japonicus for superoxide anion （O2^·-） radicals by HPLC-DAD coupled with chemiluminescence（CL） was studied and the active constituents in it were screened.The aqueous extracts of Ophiopogon japonicus were divided into the ethyl ether fraction and the n-butanol fraction.Their activities were investigated respectively and the reduction of CL with the pyrogallic acid 3amino-phthalhydrazid （luminol）-Na2CO3/NaHCO3 buffer （pH 11.0） system worked as an activity index of the antioxidant constituents.Major active components in Ophiopogon japonicus could be identified by HPLC-DAD-MS/MS and the reference substances.The antioxidant activities of different ingredients were evaluated by standard potency equations using quercetin as positive control.The experimental data indicated that the scavenging activities of Ophiopogon japonicus for superoxide anion （O2^·-） radicals are mainly from homoisoflavone in the ethyl ether fraction.It has shown that the method for on-line detection is an advantageous tool to rapidly determinate antioxidants from complex herbs.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2010年第2期141-145,共5页
Journal of China Pharmaceutical University
基金
国家自然科学基金资助项目(No.30772792
No.30973965)~~
