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干扰Gnb21l对节律基因mPer1表达的影响

Effect of Gnb2l1 on expression of circadian gene mPer1
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摘要 目的研究干扰Gnb21l表达对节律基因mPer1表达的影响。方法采用12-十四酸佛波酯-13-乙酸盐(Phorbol 12-Myristate 13-acetate,PMA)诱导NI H3T3细胞,使其节律基因稳定表达,然后用脂质体介导法将pGenesil-1/Gnb2l1质粒(实验组)及pGenesil-1/vector质粒(对照组)分别转染入NI H3T3细胞中,采用RT-PCR半定量技术检测不同时间点NTH3T3细胞中Gnb2l1及mPer1 mRNA的表达水平的变化情况。结果实验组比对照组的Gnb2l1以及mPer1 mRNA的表达量均明显降低(P<0.05),但经余弦分析比较两组的mPer1表达周期无明显改变。结论干扰Gnb2l1使节律基因mPer1表达量降低。 Objects To study the expression of the circadian gene mPer1 by interfering Gnb21l expression. Method NIH3T3 cells were induced with PMA to stably express circadian genes. And then Gnb21l interferenee-plasmid （Experimental group） and control-plasmid （Control group） were transferred into NIH3T3 cells with Lipofectamine-mediated gene transfection method. The Gnb21l and mPer1 gene expression were observed by Semi-quantitative RT-PCR. Result The express of Gnb21l was obviously interfered by Gnb21l interference-plasmid. The express of roPer1 in Experimental group was decreased compared that in Control group, but the circadian period of mPer1 was not influenced by Gnb21l interference-plasmid. Conclusion Interfering Gnb21l expression in NIH3T3 cells can decrease the expression of roPer1.
作者 谯邦兴 朱雨 刘延友 邹晏 汪宇辉 江舟 王正荣
机构地区 四川大学华西基础医学与法医学院卫生部时间生物学重点实验室
出处 《西部医学》 2010年第5期805-807,共3页 Medical Journal of West China
关键词 节律基因 Gnb21l基因 mPer1基因 转染 Circadian genes Gnb21l gene roPer1 gene Transfection
分类号 R33 [医药卫生—人体生理学]
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参考文献14

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西部医学
2010年 第5期

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