摘要
目的探讨结直肠癌组织中VEGF-C及EphA2蛋白的表达及其与结直肠癌淋巴转移的关系。方法应用免疫组化SP法检测结直肠癌组织、癌旁组织及远癌组织中VEGF-C及EphA2蛋白的表达,结合淋巴转移情况,应用SPSS13.0统计软件分析VEGF-C及EphA2蛋白与结直肠癌淋巴转移的关系。结果VEGF-C蛋白在结直肠癌中呈高表达(100%),显著高于癌旁组织(75.95%)及远癌组织(63.29%)(均P<0.01),与Dukes分期及肿瘤组织分化程度有关(P<0.01),而与年龄及肉眼类型无关(均P>0.05)。EphA2蛋白在结直肠癌组织中呈高表达(98.7%),显著高于癌旁组织(25.3%)及远癌组织(18.99%)(均P<0.01),且与Dukes分期、肿瘤组织分化程度及肉眼类型有关(P<0.05),而与年龄无关(P>0.05)。VEGF-C蛋白在结直肠癌中的表达与EphA2蛋白呈正相关(r=0.565,P=0.000)。结论VEGF-C及EphA2蛋白在结直肠癌中高表达现象与结直肠癌的淋巴转移密切相关,VEGF-C及EphA2有成为结直肠癌治疗中新靶位点的潜在可能。
Objeetive To approach the expression of vascular endothelial growth factor - C （ VEGF - C）, EphA2, the relationship between lymphatic metastasis and the expression in colorectal carcinoma. Method The expression of VEGF - C and EphA2 in eases of coloreetat carcinoma, cases of latero - carcinoma and cases of control group were determined by immunohistochemie （ SP method ）, combined with the lymphatic metastasis of colorectal carcinoma. The experi- mental data were analyzed by SPSS13.0. Results The positive expression of VEGF - C protein was found 100% in cases of colorectal carcinoma, 75.95% in cases of latero - carcinoma and 63.29% in cases of control group. There was significant correlation between the cases of coloreetal carcinoma and the cases of latero - carcinoma, between the cases of colorectal carcinoma and the cases of control group （ both P 〈0.01 ）. There was significant correlation between the expression of VEGF- C and the staging of Dukes, histological grade （both P 〈 0.01 ）. There was no significant correlation between the expression of VEGF - C and age, the shape of carcinoma （ P 〉 0.05 ）. The positive expression of EphA2 protein was found 98.7% in cases of colorectal carcinoma, 25.3% in cases of laterocarcinoma and 18.99% in cases of control group. There was significant correlation between the cases of colorectal carcinoma and the cases of latero - carcinoma, between the cases of coloreetal carcinoma and the cases of control group （ both P 〈 0.01 ）. There was no significant correlation between the expression of EphA2 and age （ P 〉 0.05 ）. There was positive correlation between the expression of VEGF - C and EphA2 in the cases of colorectal carcinoma（ r = 0. 565, P = 0. 000 ）. Conclusion There was significant correlation between the high expression of VEGF - C, EphA2 and lymphatic metastasis in tissue of colorectal carcinoma. VEGF - C and EphA2 may be a new target site in the treatment of colorectal carcinoma.
出处
《南华大学学报（医学版）》
2010年第2期214-218,共5页
Journal of Nanhua University(Medical Edition)
基金
湖南省教育厅06C705项目资助
