摘要
从天津市病猪中分离得到3株猪繁殖与呼吸综合征病毒(PRRSV),分别命名为TJ-S1、TJ-S2和TJ-S3。根据GenBank中PRRSV BJ-4株的基因序列设计并合成针对Nsp2基因高变区的引物,RT-PCR扩增目的基因并将其克隆到pGEM-T载体进行测序,并与国内外已发表的毒株序列进行比较。结果表明,3个毒株都是美洲型PRRSV,而且TJ-S1、TJ-S2株的Nsp2基因发生30个氨基酸的不连续缺失;基因系统发育树分析表明,TJ-S1株、TJ-S2株与HuN株、SD-14株的亲缘关系很近,TJ-S3株与CH-1a株的亲缘关系较近。
To study the variation of procine reproductive and respiratory syndrome virus（PRRSV）,three PRRSV isolates（TJ-S1,TJ-S2 and TJ-S3） were obtained from Tianjin province＇s pig farms.A pair of primers were designed according to the published sequence of Nsp2 gene of BJ-4 strain and used to amplify the partial Nsp2 gene by RT-PCR,the PCR products were cloned into pGEM-T vector and then sequenced.The sequence was analyzed by DNAstar software.Sequence and phylogenetic analysis showed that all these 3 isolates belong to the North American genotype,TJ-S1 strain and TJ-S2 strain shared a common feature of distinctive deletions in 221 and 272-300 amino acids.The phylogenetic tree revealed that TJ-S1 strain,TJ-S2 strain had close relationship with HuN strain and SD-14 strain,TJ-S3 was close to CH-1a.
出处
《动物医学进展》
CSCD
北大核心
2010年第4期26-29,共4页
Progress In Veterinary Medicine
基金
天津市应用基础及前沿技术研究计划项目
