摘要
为了探讨钙蛋白酶3(Calpain3,CAPN3)基因作为影响牛肉质性状候选基因的可能性,寻找与牛肉质性状相关的分子标记,对CAPN3基因的5′端区域进行SNPs检测,分析不同基因型在不同品种或者组合群体间的分布规律。利用测序和PCR-RFLP的方法进行SNPs检测和基因型分析,计算等位基因频率、各位点多态信息含量。结果发现,2546位处发生点突变C→T。各位点的3种基因型与牛生产性状的最小二乘分析结果表明,AB与BB基因型个体在胴体质量指标上存在显著差异(P<0.05)。初步推断CAPN3基因可能是影响牛肉质性状潜在的主效基因或与主效基因连锁,并且这个位点具有成为分子标记的潜在可能。
The 5＇UTR of CAPN3（Calpain3）gene was detected for searching SNPs and analyzing the distribution rule of different genotypes in different breeds,in order to study the relationship between CAPN3 gene and meat quality,meanwhile,searching the markers correlated with meat quality.SNPs were screened by sequencing and different genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）,then allele frequencies and polymorphism information content（PIC）were calculated.The result indicated that a new SNP（C→T）was found at 2 546 bp.The analysis result of the relationship between genotypes and productive performances by the Least Square Method（LSM）showed that the difference between AB and BB genotypes in carcass weight was significant（P0.05）.CAPN3 gene was primarily deduced to be a potential major gene or linked to major gene effecting meat quality traits.And this SNP might be a potential molecular marker of MAS.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第4期398-402,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
中国热带农业科学院中央级公益性科研院所基本科研业务费专项(PZS-013)
畜禽种质资源共享平台建设项目(2005DKA21101)
