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亚洲1型口蹄疫病毒L蛋白缺失株的构建及其生物学定性 被引量：2

Construction and biological characterization of leader-deleted Asia1 foot-and-mouth disease virus
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摘要 为确定口蹄疫病毒(FMDV)L蛋白缺失对其生物学特性的影响,本研究在已建立的Asia1型FMDV反向遗传操作平台基础上,利用融合PCR方法缺失L蛋白基因编码区,构建了缺失L蛋白的重组Asia1型FMDV(rFMDV-ΔL)。拯救病毒的一步生长曲线结果表明,rFMDV-ΔL与亲本病毒相比在BHK-21细胞中的复制水平下降10倍,乳鼠毒力试验表明rFMDV-ΔL的毒力比亲本毒株下降6倍。另外,rFMDV-ΔL在BHK-21细胞上产生病变的速度明显变慢,形成蚀斑的时间延长,致乳鼠死亡所需时间也比亲本毒株明显延长。本研究是关于亚洲1型FMDVL蛋白结构与功能研究的首次报道,为亚洲1型FMDVL蛋白结构与功能的研究奠定了基础。 By using overlapping PCR,a recombinant food and mouth disease virus（FMDV） with L gene delated（rFMDV-ΔL） was generated based on FMDV Asia1 reverse genetic system. Comparing with parental virus,the replication of rFMDV-ΔL was 10-fold lower in BHK-21 cells,and pathogenicity to mice was reduced by 6-fold. Moreover,there was a delay in spread of the rFMDV-ΔL in mice,consistent with the observation that virus replication in virus-infected BHK-21 cells were delayed relative to wild-type virus. This is the first report on studies of the structure and function of the leader（L） proteinase of serotype Asia1 FMDV.
作者 薛美 王海伟 于力
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2010年第4期259-262,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 黑龙江省科技攻关重大项目专题(GA06B202)
关键词 ASIA1型口蹄疫病毒 L蛋白缺失 重组病毒拯救 生物学定性 foot-and-mouth disease virus serotype Asia1 deletion of leader （L） proteinase rescue recombinant virus biological characterization
分类号 S852.65 [农业科学—基础兽医学]
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参考文献9

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二级参考文献13

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中国预防兽医学报
2010年 第4期

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