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姜黄素对HepG2细胞固醇调控元件结合蛋白-2表达的影响 被引量：1

The effect of curcumin on the expression of SREBP-2 in HepG2
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摘要 目的研究姜黄素对人肝癌细胞株(Human hepatocellular liver carcinoma cell line,HepG2)固醇调控元件结合蛋白-2(sterol regulatory element-binding protein-2,SREBP-2)基因表达的影响,从信号调控通路水平进一步探讨姜黄素的降胆固醇作用机理。方法分别利用蛋白免疫印迹和实时荧光定量聚合酶链反应(Polymerase Chain Reactionm,PCR)技术研究姜黄素对HepG2细胞SREBP-2核片段碱性螺旋-环-螺旋蛋白(basic helix-loop-helix protein,bHLH)表达及SREBP-2 mRNA的影响。结果20 mol/L姜黄素可以促进HepG2细胞SREBP-2核片段bHLH增多,而对SREBP-2mRNA表达无影响。结论姜黄素可以通过促进HepG2细胞SREBP-2核片段bHLH的增多,上调低密度脂蛋白受体(low density lipoprotein receptor,LDL-R)的表达,起到降低血清胆固醇的作用。 Objective To investigate the effects of curcumin on SREBP-2 expression in the human hepatoma cell line HepG2 and find out the mechanism of curcumin in lower-cholestrol at the SREBPs regu- lation pathway level. Methods Western blotting and Real time PCR was used to identify the effect of curcumin on the expression of SREBP-2 in the HepG2. Results The nuclear segment of active SREBP-2 （bHLH） was elevated after 20 mol/L curcumin treatment in the HepG2 by Western blotting. Conclusion Curcumin up-regulates the expression of the SREBP-2 in the HepG2.
作者 窦晓兵 陈庆 王宇浩 豆敏华 范春雷
机构地区 浙江中医药大学生命科学学院
出处 《环球中医药》 CAS 2010年第3期183-186,共4页 Global Traditional Chinese Medicine
基金 浙江省自然科学基金(Y2090891) 浙江省新苗人才计划项目(2008R40G2120015) 浙江中医药大学资助项目(17108016)
关键词 姜黄素 人肝癌细胞株 固醇调控元件结合蛋白-2 碱性螺旋-环-螺旋蛋白 Curcumin HepG2 SREBP-2 bHLH
分类号 R285.5 [医药卫生—中药学]
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参考文献12

  • 1窦晓兵,沃兴德,范春雷,俞颖,钱颖,黄小黎,凌霞.姜黄素对人肝细胞HepG2中低密度脂蛋白受体基因表达影响的研究[J].中国药学杂志,2007,42(8):572-575. 被引量：9
  • 2Xiaobing Dou,Chunlei Fan,Like Wo,,et al.Curcumin upregulates LDL receptor expression via sterol regulatory element pathway in HepG2 cells[J].Planta Med,2008,74:1-6.
  • 3钱颖,范春雷,窦晓兵,胡林峰,沃兴德.姜黄素对293细胞固醇调控报告系统的作用[J].中国药理学通报,2006,22(7):828-830. 被引量：11
  • 4Dieter Peschel,Ramona Koerting,Norbert Nass.Curcumin induces changes in expression of genes involved in cholesterol homeostasis[J].Journal of Nutritional Biochemistry,2007,18(2):113-119.
  • 5Chunlei Fan,Xingde Wo,Ying Qian,et al.Effect of curcumin on the expression of LDL receptor in mouse macrophages[J].J Ethnopharmacol,2006,105(1-2):251-254.
  • 6Brown MS,Goldstein JL.A receptor-mediated pathway for cholesterol homeostasis[J].Science,1986,232-234.
  • 7Brown M S,Goldstein J L.The SREBP pathway:regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor[J].Cell,1997,89:331-340.
  • 8Wang X,Sato R,Brown MS,et al.SREBP-1,a membrane-bound transcription factor released by sterol regulated proteolysis[J].Cell,1994,77(1):53-62.
  • 9Arun Radhakrishnan,Yukio Ikeda,Hyock Joo Kwon,et al.Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi:Oxysterols block transport by binding to Insig[J].Proc Natl Acad Sci,2007,104(16):6511-6518.
  • 10Briggers,M.R,Yokoyama C,Brown,M S,et al.Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter[J].J Biol Chem,1993,268(19):14490-14496.

二级参考文献37

共引文献36

同被引文献35

  • 1Bauer S, Wanninger J, Schmidhofer S, Weigert J, Neumeier M, Dora C, Hellerbrand C, Zimara N, Schaffler A, Aslanidis C and Buechler C, 2011. Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes. Endocrinology, 152( 1): 26-35.
  • 2Bhatt S P, Nigam P, Misra A, Guleria R, Luthra K, Vaidya M, Jain S K and Pasha M A, 2011. SREBP- 2 1784 G/C genotype is associated with non-alcoholic fatty liver disease in north Indians. Disease Markers, 31 (6): 371-377.
  • 3Brown M S and Goldstein J L, 1997. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell, 89(3): 331-340.
  • 4Edwards P A, Tabor D, Kast H R and Venkateswaran A, 2000. Regulation of gene expression by SREBP and SCAP. Biochimica et Biophysica Acta, 1529(1-3): 103-113.
  • 5Goldstein J L, Rawson R B and Brown M S, 2002. Mutant mammalian cells as tools to delineate the sterol regulatory element- binding protein pathway for feedback regulation of lipid synthesis. Archives of Biochemistry and Biophysics, 397(2): 139-148.
  • 6Hasegawa S, Noda K, Maeda A, Matsuoka M, Yamasaki M and Fukui T, 2012. Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP- 2 and affects serum cholesterol levels. Molecular Genetics and Metabolism, 107 (3): 553-560.
  • 7He T C, Zhou S, Da C L, Yu J, Kinzler K W and Vogelstein B, 1998. A simplified system for generating recombinant adenoviruses. Proceedings of the National Academy of Sciences of the USA, 95(5): 2509-2514.
  • 8Hitt D C, Booth J L, Dandapani V, Pennington L R, Gimble J M and Metcalf J, 2000. A flow cytometric protocol for titering recombinant adenoviral vectors containing the green fluorescent protein. Molecular Biotechnology, 14 (3): 197-203.
  • 9Horton J D, Cohen J C and Hobbs H H, 2009. PCSK9: A convertase that coordinates LDL catabolism. Journal of Lipid Research, 50(Suppl S): 172-S177.
  • 10Horton J D, Goldstein J L and Brown M S, 2002. SREBPs: Activators of the complete program of cholesterol and fatty acid synthesis in the liver. Journal of Clinical Investigation, 109(9): 1125-1131.

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环球中医药
2010年 第3期

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