摘要
应用原位杂交法和放射免疫测定法，观察P物质（SP）及福尔马林对脊髓前生长抑素原（PPS）mRNA表达和SP样免疫活性物（Ir－SP）含量的影响。结累表明足底注射福尔马林150μl，脊髓PPS－mRNA1h开始升高，2h明显增多，4h达高峰。同时Ir－SP的含量1h增多，2h稍有下降。大鼠鞘内注射SP1h，脊髓PPS－mRNA变化不明显，2h明显增多，4h达高峰。说明SP及伤害性刺激可促使SOM转录、合成。
In the present study the authors used in situ hybridization and RIA to observe the effect of Substance P(SP)and formalin on prepro-somatostatin mRNA (PPS-mRNA)expression and contens of immunoreactive SP(Ir-SP) in th spinal cord of rats. One hour after injection of formalin in the right hindpaw of the rats. the PPS-mRNA and Ir-SP began to increase in the spinal cord. Two hours after injection, the PPSmRNA increased remarkedly and reached its maximal level in four after intrathecal injection of SP, the PPS-mRNA also increase, and reached its maximal level four hours after injection. It is suggested that SP and noxious stimulation induced an increase of SOM transcription and synthesis.
关键词
生长抑素
MRNA
P物质
福尔马林
脊髓
prepro-somatostatin mRNA
substance P
formalin
in situ hybridization
spinal cord
