摘要
目的应用DNA芯片杂交技术快速检测临床分离株中结核分枝杆菌对异烟肼(INH)、利福平(RIF)、链霉素(SM)和乙胺丁醇(EMB)的耐药性,并评价其临床应用价值。方法采用DNA芯片技术,将固定于硝酸纤维素膜上的四种药物常见耐药基因的特异性探针,与结核分枝杆菌临床分离株生物素标记的聚合酶链反应产物进行杂交,共对97株临床分离株进行检测,并将结果与绝对浓度法药敏试验结果进行比较。结果 INH、RIF、SM和EMB的耐药基因检出的灵敏度分别为89.6%、98.6%、81.8%和88.2%,特异度分别为93.3%、100%、100%和100%。结论简便快速敏感的DNA芯片杂交方法适用于临床批量结核分枝杆菌耐药性的初筛。
Objective To study the rapid detection of INH, RIF, SM and EMB in myeobacterium tuberculosis by DNA chip and to assess its value in clinical applications. Methods The amplified PCR fragments labeled biotin were used to hybridize with membrane - bound specific oligonucleotide probes. Total 97 strains of MTB were analyzed,and the results were compared with conventional drug suscep- tibility tests. Results The sensitivity of INH, RIF, SM and EMB was 89. 6% ,98.6% ,81.8 % and 88.2%, and specificity was 93.3 %, 100% ,100% and 100% respectively. Conclusion High sensitivity,short turnaround times and the potential for screening large numbers of specimens rapidly, make the DNA chip technique suitable as a first-line screening assay for drug resistant TB.
出处
《临床肺科杂志》
2010年第6期825-828,共4页
Journal of Clinical Pulmonary Medicine
基金
国家十一五科技重大专项(艾滋病和病毒性肝炎等重大传染病防治)(2009ZX10005-018)
关键词
结核
分枝杆菌
核酸杂交
药物耐受性
突变
mycobacterium
tuberculosis
nuleotide hybridization
drug resistance
mutation
