摘要
土壤微生物在地球化学循环、土壤有机质周转以及土壤肥力与质量方面发挥重要作用。选取双季稻区6种主要的水稻土类型,应用磷脂脂肪酸(PLFAs)分析方法,研究土壤类型对土壤微生物的结构和多样性的影响。结果表明,6种不同的土壤类型中共检测出了21种不同的磷脂脂肪酸类型,其中紫色土磷脂脂肪酸总量最高,达到107.05ngg-1干土,河沙泥磷脂脂肪酸含量最低,为59.75ngg-1干土。从革兰氏阳性菌、革兰氏阴性菌、真菌及相互间的比值变化看,6种土壤类型间差异较大。主成分分析结果表明,第一主成分和第二主成分可以解释总变异的76.7%,除C16:0外,大部分的非饱和脂肪酸和环式脂肪酸的变异可以在第一主成分中反映出来,第二主成分主要反映含羟基的脂肪酸变异。
Soil microbial communities play a central role in global biogeochemical cycle and soil organic transformation, according to the effect on the soil fertility and soil health. The objective of this study was to use phospholipid fatty acid （PLFA） profiles to characterize and identify microbial community structure among six paddy soil types. The results indicated that total twenty one PLFA （phospholipid fatty acid） profiles were detected among the six soil types, purple type had the highest content of fatty acid, with the value of 107.05 ng g^-1, and the lowest one was in river type whose value was 59.75 ng g^-1. The amounts of Gram-positive bacteria, Gram-negative bacteria, fungi were different among the six soil types, and the ratio between of them was also different. Principal component analyses of whole soil were conducted to determine microbial community structure of the 21 PLFAs detected in the six soil types, the main two principal components explained 76.7% of the total variance. All unsaturated PLFAs except C16：0 were highly expressed in the first principal component, the OH fatty acid were expressed in the second principal component.
出处
《作物学报》
CAS
CSCD
北大核心
2010年第5期826-832,共7页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30671222
30871491)
国家"十一五"科技支撑计划项目(2007BAD89B01
2006BAD02A15)
国家公益性行业(农业)科研专项(200803028)资助
关键词
水稻
土壤类型
土壤微生物
磷脂脂肪酸
Rice Soil type Soil microbial communities Phospholipid fatty acids（PLFA）
