摘要
目的通过体外抗肿瘤实验考察白首乌苷对多种瘤株的抑制作用。方法采用体外MTT比色法,结合生长曲线法及细胞形态学观察研究白首乌苷对B-16、K-562、PC-3和HeLa肿瘤细胞增殖的抑制作用。结果白首乌苷对B-16、K-562、PC-3和Hela肿瘤细胞均有较强的体外细胞毒作用,半数抑制浓度(IC50)分别为(10.0±0.4),(23.1±1.3),(34.8±2.4),(41.6±6.4)μg.mL-1,且具有明显剂量-效应关系;生长曲线表明,白首乌苷对PC-3肿瘤细胞的作用随着给药浓度增加而增强,且在一定剂量范围内随时间增加其对肿瘤细胞的作用增强,具有明显的量-效和时-效关系;观察细胞形态发现,白首乌苷能使细胞密度明显降低,细胞皱缩,部分悬浮,细胞碎片增多。结论白首乌苷对多种体外生长的肿瘤细胞均有抑制作用,且有一定的选择性。
ObjectiveTo separate and purify the glycosides from the root of Cynanchum auriculatum Royle ex Wight and investigate the anti-tumor effect of it on the multiform tumor cells. MethodsThe anti- proliferation effects of glycosides on B-16、K-562、PC-3 and Hela tumor cells were studied by MTT assay,growth curve methods and cell morphological observation. ResultsGlycosides of Radix Cynanchi Auriculati had potent cytotoxic effect on all four tumor cells. The 50% inhibiting concentration （IC50） of which were （10.0±0.4）,（23.1±1.3）,（34.8±2.4）,（41.6± 6.4） μg·mL^-1 respectively,showing evident dose-effect relationship. It was indicated that the anti-tumor effect of glycosides on PC-3 tumor cells enhanced as the concentration increased at a certain time and as the time increased in a certain range of dosage by growth curve methods. The glycosides of Radix Cynanchi Auriculati significantly inhibited the growth of tumor cells,reduced density of tumor cells in culture media,where some cells crimpled,some suspended,and some decomposed into fragments. ConclusionThe glycosides of Radix Cynanchi Auriculati could inhibit the growth of many kinds of tumors in vitro and was selective to certain kinds of tumors.
出处
《医药导报》
CAS
2010年第5期582-584,共3页
Herald of Medicine
关键词
白首乌苷
抗肿瘤作用
体外
Glycosides of Radix Cynanchi Auriculati
Anti-tumor activity,in vitro
