摘要
目的用大蒜新素治疗鼠巨细胞病毒(murine cytomegalovirus,MCMV)播散性感染小鼠至慢性期,检测小鼠唾液腺、肝、脾和肾组织内MCMV DNA载量动态变化,在整体水平上观察大蒜新素抗MCMV的长期疗效。方法BALB/c幼鼠60只,腹腔接种MCMVSmith株建立MCMV播散性感染模型,随机分为大蒜新素治疗组和模拟治疗组各30只,接种病毒后24 h,两组分别腹腔注射大蒜新素25 mg.kg-1.d-1和0.9%氯化钠注射液0.2 mL,qd,共120 d。于治疗后1,3,7,14,28,45,60,75,90和120 d,每组随机选择小鼠3只,取唾液腺、肝、脾和肾组织,采用real time PCR法检测组织内MCMV DNA载量。结果模拟治疗组小鼠唾液腺内MCMV DNA载量最高,在治疗后14 d(感染后15 d)达高峰;肝、脾和肾内病毒DNA载量治疗后7 d达峰值;治疗28 d(感染29 d)后,各组织内病毒DNA载量明显减少,尤其是肝、脾和肾组织病毒DNA呈持续低水平(进入慢性期)。与模拟治疗组比较,大蒜新素治疗组治疗后7,14和28 d唾液腺、脾和肾内MCMVDNA载量显著降低;治疗后7和14 d肝内病毒DNA载量明显降低。进入慢性期后,两组小鼠各脏器病毒DNA载量差异无显著性。结论大蒜新素长期治疗不能完全清除CMV,但在急性感染期(病毒增殖高峰期)能显著降低脏器内病毒DNA载量。
ObjectiveTo investigate the long-term therapeutic effect of allitridin against cytomegalovirus in vivo by measuring the dynamic changes of cytomegalovirus （MCMV） DNA loads in salivary gland, liver, spleen and kidney of mice with disseminative infection of murine cytomegalovirus. Methods60 BALB/c young mice were inoculated peritoneally with stocks of Smith strain MCMV to establish a MCMV disseminative infection model and then randomly and equally allocated into allitridin-treated group and mock-treated group （30 mice in each group）. After 24 h of viral infection, the model mice from both allitridin-treated group and mock-treated group were peritoneally injected with allitridin （25 mg·kg-1·d-1,0.2 mL） and nomal saline （0.2 mL） respectively, once a day, for totally 120 days. At 1 d, 3 d, 7 d, 14 d, 28 d, 45 d, 60 d, 75 d, 90 d and 120 d after treatment, 3 mice in each group were randomly sacrificed for quantitatively detecting the load of MCMV DNA in their salivary gland, liver, spleen and kidney tissues by using a real time PCR method. ResultsIn the mock-treated group, MCMV DNA levels were the highest in salivary glands and reached the peak at 14 d after treatment （15 d post infection）, and peaks at 7 d after treatment in liver, spleen and kidney tissues. After 28 d of treatment （29 d post infection）, MCMV DNA levels were obviously reduced in each organ of mock-treated group, especially in liver, spleen and kidney, indicating it entered into chronic infection phase. Compared to the mock-treated group, alliridin therapy significantly reduced MCMV DNA levels in salivary gland, spleen and kidney tissues at 7 d, 14 d and 28 d after treatment and in liver tissues at 7 d and 14 d post treatment. During the chronic infection phase （after 45 d of treatment）, MCMV DNA levels in each organ of both groups were no significant different. ConclusionAllitridin for long-term treatment didn’t completely eliminate CMV viruses, but in acute infection phase （viral reproduction peak phase）, it could significantly reduce the viral load.
出处
《医药导报》
CAS
2010年第5期561-565,共5页
Herald of Medicine
基金
国家自然科学基金资助项目(基金编号:30572345)
