摘要
利用RT-PCR技术从感染温州蜜柑萎缩病毒的叶片中扩增出SDV大外壳蛋白(Large coat protein,CPL),将CPL克隆到pGEM-T载体后进行测序分析。DNA序列分析表明,SDV FJ的CPL基因cDNA序列全长为1329bp,编码443个氨基酸。与同属其他病毒序列对比分析结果显示,所得序列与日本报道的SDV S-58株系核苷酸和氨基酸同源性最高,分别为98.1%和98.6%。核苷酸序列与S-58相比25处发生了替换突变,其中T和C之间的替换频率最高,占绝对优势。
The large coat protein （CPL） gene of Satsuma dwarf virus Fengjie isolate （SDV FJ） was amplified by RT-PCR and cloned into vector pGEM-T. Sequence analysis showed that the cDNA of SDV-FJ CPL was 1 329 bp, encoding a protein of 443 amino acids. Comparison of the sequence with those from other viruses of genus Sadwavirus showed high identities （98.1% and 98.6%） of nucleotides and amino acids between SDV FJ and SDV S-58 reported in Japan. Totally, 25 nucleotide differences were found between the CPL of SDV FJ and SDV S-58, among which the mutations between T and C account for a high percentage.
出处
《果树学报》
CAS
CSCD
北大核心
2010年第3期457-460,共4页
Journal of Fruit Science
基金
重庆市自然科学基金(CSTC.2007BB1349)
西南大学博士启动基金(SWUB2006042)
