摘要
从浙江仙居某制药厂废水处理站的活性污泥中驯化、分离得到1株能以雌二醇(17β-estradiol,E2)为唯一碳源生长的细菌菌株E2-Y.经过对其形态特征、生理生化以及16SrDNA序列分析,确定该菌为一种芽孢杆菌(Bacillussp.).通过摇瓶实验考察了温度、pH等因素对E2微生物降解性能的影响,得出最适温度为30℃,最佳pH值7.5.培养基中不同添加物对E2降解的影响迥异:蛋白胨、牛肉膏等有较强的促进作用;Ba2+、Zn2+、Sn2+、Cd2+、Cr2+、Pb2+等几种金属离子的抑制作用依次增强;而投加硝酸钾、淀粉、氯化铵、Ca2+、Fe2+等物质对E2降解无明显影响.在最佳实验条件下,该菌株可以在7d内将初始浓度为0.5～50mg/L的E2完全降解,E1是菌株E2-Y降解E2的最初代谢产物,并且可进一步被逐渐降解;降解实验进行15d后,初始浓度为1mg/L的E2的雌激素总体效应可被该菌株降低95%以上.
A novel bacterium capable of degrading 17β-estradiol （E2） was isolated from the activated sludge collected from wastewater treatment plant of an oral contraceptives producing factory in Xianju,Zhejiang. According to its morphology,physiochemical characteristics and 16S rDNA sequence analysis,this strain was identified as Bacillus sp. The optimal pH and temperature for E2 biodegradation in shaking flasks were 7. 5 and 30℃,respectively. The enhanced action of peptone and Lab-Lemco were quite obvious; metal ions such as Ba^2 ＋ ,Zn^2 ＋ ,Sn^2 ＋ ,Cd^2 ＋ ,Cr^2 ＋ ,Pb^2 ＋ inhibited degradation,and the actions were ordinal increasing; the addition such as Ca^2 ＋ ,Fe^2 ＋ ,KNO3 and amylum had no obvious effect on the degradation of E2. Furthermore,this strain could degrade E2 completely within 7 days with initial concentration ranged from 0. 5 to 50 mg/L and was capable of converting E2 to estrone （E1）,then to nonestrogenic compounds,thus 95% of initial estrogenic activities was decreased within 15 days during the biodegradation process.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2010年第5期1313-1319,共7页
Environmental Science
基金
浙江省自然基金项目(Y506237)
浙江省科技厅分析测试项目(2008F70014)
浙江工业大学重中之重学科开放基金研究课题项目(20080212)
关键词
17Β-雌二醇
芽孢杆菌
环境雌激素
降解特性
雌激素活性
17β-estradiol （E2）
Bacillus sp.
environmental estrogen
degradation characteristics
estrogenic activities
