摘要
目的:探讨博来霉素致肺纤维化大鼠各时期支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α(TNF-α)的动态表达和意义。方法:90只雄性Wistar大鼠随机分为对照组、疾病模型组和甲泼尼龙治疗组。对照组给予生理盐水,而疾病组和治疗组给予博来霉素溶液(5 mg/kg)一次性气管内注入,对照组和疾病组于造模当天腹腔注射生理盐水(每只0.3 mL),以后每天1次,治疗组于造模当天腹腔注射甲泼尼龙(15 mg/kg),以后每天1次,后于第1,3,7,14,21,28天腹主动脉放血处死各组大鼠各5只,取BALF,应用ELISA法测定BALF中TNF-α的含量,并计数白细胞总数和中性粒细胞百分比。结果:BALF中疾病组TNF-α、白细胞总数、中性粒细胞百分比在各时间点较正常组升高,且TNF-α在第14天达到高峰,而白细胞总数和中性粒细胞百分比分别于7 d,14 d达峰值,以后逐渐下降,第28天仍高于正常组,有统计学差异(P<0.05)。治疗组TNF-α、白细胞总数、中性粒细胞百分比较模型组有所下降,差异有显著性(P<0.05),但仍高于正常组,差异有统计学意义(P<0.05)。结论:肺纤维化发生的机制可能与TNF-α的过度表达有关,并依其表达水平可以推断疾病的进程。甲泼尼龙通过其抗炎作用在大鼠肺损伤早期阶段是一种有效的防治药物。
Objective： To investigate the dynamic expression and significance of TNF-α in bronchoalveolar lavage fluid（BALF） in mice with blemycin induced pulmonary fibrosis at various periods. Methods：90 Wistar mice were randomly divided into three groups ：control group ,disease model group and prednislone treated group. Control group was poured by saline. Disease group and therapic group were poured by bleomycin solutions. The control group and the disease group were injected with saline in the abdomen from the first day then once everyday,the therapic group were also injected with prednislone in abdomen from the first day（15 mg/kg） then once everyday. Killing five mice from every group on the 1 st ,3rd,7th, 14th ,21th and 28th day. Counting the white blood total and the percentage of nertral granalar. ELASA method was used to analyse the content of TNF-α in BALF. Results：The content of TNF-a,the white blood total and the percentage of nertral granalar from disease group in BALF were higher than thoseof control group at various periods. TNF-α peaked on 14th day and white blood total and the percentage of nertral granala peaked on 7th and 14th day,then began to deceased but still higher than those of control group,it hasthe statistics difference（P〈0.05）. The three group numbers from treated group in BALF were little lower than those of disease group. The difference is obviously （P〈0.05） ,but still higher than those of control group,it has statistics difference （P 〈0.05）. Conclusion：The excessive expression of TNF-a maybe the resson for the pulmonary fibrosis. By which the progression and activities of pulmonary fibrosis can be judged. It concludes that prednisone resisted its inflammatory reflection. It indicated that prednislone was the valid protection and treatment drug in the pulmonary injury through its resisted inflammatory reflection.
出处
《临床医药实践》
2010年第5期330-333,共4页
Proceeding of Clinical Medicine
