摘要
堇叶碎米荠(Cardamine violifolia)是在我国富硒地区发现的一种富集硒植物,具有很高的科学研究价值。研究以堇叶碎米荠的幼叶为外植体,进行组织培养研究,结果表明:诱导愈伤组织的最佳培养基为MS+6-BA1.0mg/L+2,4-D0.6mg/L,诱导率高达85%,以培养基MS+6-BA1.0mg/L+NAA0.6mg/L诱导不定芽分化效果最好;在生根阶段以培养基MS+NAA1.0mg/L生根效果最好,生根3.2条/株,根系健壮。
Cardamine violifolia is a selenium-rich plant found in the highselenium-contained area of China,which has a high scientific research value. Taking eardamine violifolia＇ s young leaves as explants, the researchers conducted tissue culture studies. The results showed that inducing the best medium of callus was MS ＋ 6-BA 1.0 mg/L ＋ 2,4-D 0.6 mg/L, with a induction rate as high as 85%. The medium MS ＋ 6-BA1.0 mg/I ＋ NAA 0.6 mg/L has the highest effects of adventitious bud differentiation, in rooting phase, the medium MS ＋ NAA 1.0 mg/L has the best rooting effect, with robust root of 3.2 / plant.
出处
《贵州师范大学学报（自然科学版）》
CAS
2010年第2期10-12,共3页
Journal of Guizhou Normal University：Natural Sciences
基金
国家自然科学基金(批准号:40473048)
关键词
堇叶碎米荠
外植体
组织培养
cardamine violifolia
explant
tissue culture
