摘要
目的探讨Ph染色体和bcr-abl mRNA在慢性粒细胞白血病诊断、分期、治疗及微小残留病变监测中的意义。方法同时检测437份慢粒标本的Ph染色体和bcr-abl mRNA的表达情况。结果慢性期、加速期和急变期的bcr-abl mRNA定量结果逐渐增高(P<0.05);异基因造血干细胞移植后的bcr-abl mRNA定量结果随时间逐渐降低,一般在移植6月后转阴,服用格列卫与异基因造血干细胞移植病程相似,但融合基因转阴所用时间较长。结论实时定量PCR技术检测bcr-abl mRNA较Ph染色体的检测更为敏感,对于疾病分期、评价疗效、监测微小残留病变以及预测疾病进展具有重要的临床应用价值。
Objective To evaluate the significance of Ph chromosome and bcr-abl mRNA expressions in diagnosis,staging and treatment of chronic myeloid leukemia（CML） as well as in monitoring minimal residual disease.Methods Expressions of Ph chromosome and bcr-abl mRNA of 437 CML patients were detected at the same time.Results Expression of bcr-abl mRNA gradually increased in blastic phase（BP）,accelerated phase（AP） and chronic phase（CP）（P〈0.05）.Quantification of bcr-abl mRNA decreased gradually after allotransplantation in the patients,and it became normal after 6 months＇ treatment.However,quantification of bcr-abl mRNA in imatinib mesylate-treated patients did not become normal until longer time later.Conclusion RT-PCR quantitative detection of bcr-abl mRNA is more sensitive than detection of Ph chromosome,and has an important clinical value in staging CML,monitoring treatment outcome,detecting the minimal residual disease and predicting disease development.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第3期325-327,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
河南省社会公益项目预研专项课题(No.072103810503)~~
