摘要
目的:观察脉冲磁场对去势大鼠血清碱性磷酸酶(ALP)变化规律及骨髓源性破骨样细胞(OLC)形成的影响。方法:3月龄Wistar雌性大白鼠18只,分为A组(伪去势组)、B组(双卵巢切除+脉冲电磁场治疗组)和C组(双卵巢切除组)。B组置电磁场仪处进行治疗3个月。眼眶取血测ALP,并对骨髓细胞进行培养,2周后对培养细胞进行抗酒石酸酸性磷酸酶(TRAP)染色并计数。结果:C组ALP值与A组及B组比较有明显升高(P<0.05),B组ALP值与A组比较虽有差别,但无统计学意义。破骨细胞计数C组与A组比较有显著差异(P<0.01),C组与B组比较有明显差异(P<0.05)。结论:脉冲电磁场能使去势大鼠血清ALP水平降低和骨髓体外培养破骨细胞形成减少。
Objective： To study the effect of pulsed electromagnetic fields（PEMs） on the changes of serum alkaline phosphatase（ALP） level and the formation of bone marrow osteoclast-like cells cultured in vitro in ovariectomized（OVX） rats.Methods： Eighteen three-month-old female Wistar rats were divided randomly into group A（sham operation）,group B（OVX ＋ PEMs） and group C（OVX only）.The rats in group B were exposed to PEMs for 3 months.The blood was obtained from their eyes,and the serum ALP was detected.The bone marrow of rats was cultured after 3 months of operation.After 2 weeks of exposure,in order to observe the changes of osteoclasts and count their numbers,the cells were stained with tartrate-resistance acid phosphatase（TRAP） method.Results： The serum ALP levels in group C were significantly higher than those in group A and B（P〈0.05）.There was no significant differences in the serum ALP levels between group A and B（P〉0.05）.The number of osteoclasts in group C was significantly higher than that in group A（P〈0.01） and group B（P〈0.05）.Conclusion： The PEMs can decrease the serum ALP level and reduce the formation of bone marrow osteoclasts cultured in vitro in ovariectomized rats.
出处
《西北国防医学杂志》
CAS
2010年第2期108-110,共3页
Medical Journal of National Defending Forces in Northwest China
基金
全军医药卫生"十五"重点课题资助项目(01z008)
关键词
脉冲电磁场
去势大鼠
碱性磷酸酶
破骨细胞
Plused electromagnetic fields
Ovariectomized rats
Alkaline phosphatase
Osteoclast
