摘要
目的研究色霉素对MPP+诱导凋亡的多巴胺能神经元的保护作用。方法体外培养的胎鼠腹侧中脑神经元,以MPP+引起多巴胺能神经元凋亡作为帕金森病的细胞模型,Hoechst 33258荧光核染色法检测神经元的凋亡情况,免疫细胞化学方法检测多巴胺能神经元内磷酸化Tau水平。结果10μmol·L-1 MPP+可以升高Tau磷酸化水平,诱导神经元发生典型的凋亡。而色霉素通过抑制Tau磷酸化,减少神经元凋亡,使TH-阳性细胞数目增加。结论色霉素可以通过抑制Tau磷酸化而对MPP+诱导凋亡的多巴胺能神经元发挥保护作用,色霉素可能用于临床防治帕金森病等神经退行性疾病。
Aim To study the effect of chromomycin on MPP＋-induced dopaminergic neuronal apoptosis.Methods An apoptosis model induced by 1-methyl-4-phenylpridnium ion（MPP^＋）was established in cultured fetal mesencephalic neurons in vitro.Dopaminergic neuronal apoptosis was detected by Hoechst 33258 staining and phospho-Tau levels were detected by immunofluorescence.Results 10 μmol·L^-1 MPP＋ hyperphosphorylated Tau at Ser396 and induced dopaminergic neuronal apoptosis.Chromomycin increased the number of TH-positive cells via inhibiting Tau phosphorylation.Conclusion These results indicate thatchromomycin inhibits Tau phosphorylation at Ser396 and therefore protects dopaminergic mesencephalic neurons from apoptosis induced by MPP^＋,which suggests that Chromomycin may be used to cure neurodegenerative disorders such as Parkinson’s disease in clinic.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第4期522-526,共5页
Chinese Pharmacological Bulletin
基金
广东省自然科学基金团队项目(No9351008901000003)
广东省教育厅教育专项--南方医科大学优秀中青年科技人才库基金资助项目(NoC1031038)
