摘要
目的:利用双向电泳技术对志贺菌敏感株与诱导耐多药株全菌蛋白进行蛋白质组学比较,寻找耐多药相关蛋白。方法:采用四类抗生素的次抑菌浓度对临床分离鉴定志贺菌敏感株进行诱导耐多药试验;对志贺菌敏感株及诱导耐多药株全菌蛋白进行双向电泳;电泳图谱采用Image Master 2D Platinum软件分析。结果:成功获得志贺菌诱导耐多药株,在志贺菌敏感株与耐多药株全菌蛋白质图谱中分别检测出946±37个和1 096±189个蛋白质斑点;经分析共发现48个差异表达的蛋白点。结论:蛋白质双向电泳技术可以成功应用于志贺菌耐多药相关蛋白的筛选,此图谱为进一步研究细菌耐多药机理奠定基础。
Objective：In order to search for the new proteins related to multidrug resistance,the proteomics of whole cellular proteins between sensitive strain and induced anti-multidrug strain of shigella flexneri by using of Two-dimensional electrophoresis（2-DE） were compared.Methods：Clinical sensitive shigella flexneri strain was induced into anti-drug strain by 1/2 MIC induced trials of four kinds of antibiotic;Immobilized pH gradient（IPG） Two-dimensional electrophoresis was adopted and the gels were analyzed by Image Master 2D Platinum software;Matrix assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF MS） were used to analyzed differential expression proteins.Results：Induced anti-multidrug strain of shigella flexneri was obtained successfully.It was found that there were 946±37 protein spots in whole cellular protein 2-DE gels of sensitive strain and 1 006±189 protein spots in that of induced anti-multidrug strain,48 differential expression protein spots were found.Conclusion：Two-dimensional electrophoresis coula be used to screen multidrug resistant protein of shigella flexneri,and this will be a basic data of mechanism of multidrug resistance.
出处
《河南医学研究》
CAS
2010年第1期1-3,共3页
Henan Medical Research
基金
河南省医学科技公关项目资助课题(WKJ2007-2-024)
关键词
志贺菌
耐多药
蛋白质组学
双向电泳
差异表达
shigella flexneri
multidrug resistance
proteomics
two-dimensional electrophoresis
mass spectrum analysis
different expression
