摘要
目的:探讨大鼠缺血再灌注后溶酶体组织蛋白酶D(Cathepsin D CD)、caspase-9在不同时段蛋白质及mRNA表达变化。方法:将60只S D大鼠随机分为三组:正常组(10只),假手术组(10只),脑缺血再灌注组(40只),线栓法制备大脑中动脉梗死模型(MCAO),免疫组化及RT-PCR法分别检测Cathepsin D、caspase-9的蛋白和mRNA表达。结果:与正常组和假手术组比较,模型组大鼠脑缺血再灌注损伤后6h Cathepsin D的蛋白和mRNA表达明显增强(P<0.05),24h达高峰,48h仍保存高水平。caspase-9蛋白和mRNA6h开始明显升高,12h达高峰,此后缓慢下降,但48h组仍显著高于对照组(P<0.05),结论:Cathepsin D、caspase-9在大鼠脑缺血再灌注后表达增强,溶酶体可能参与了脑缺血再灌注损伤后神经细胞凋亡的过程。
Objective：To study the changes of the protein and mRNA of Cathepsin D and caspase-9 expression in different time point in rat after cerebral ischemia-reperfusion. Methods：60 SD rats were randomly divided into three groups： normal group （n=10）, sham-operation group （n=10）, cerebral isehemia-reperfusion group （n=40）, prepared by suture middle cerebral artery infarction model （MCAO）, the expression of the protein and mRNA of Cathepsin D and caspase-9were respectively evaluated by methods of immunohistoehemistry and RT-PCR. Results：compared with normal group and sham-operated group , The expression level of the protein and mRNA of Cathepsin D and easpase-9 in the rats of model group increased significantly 6 hour ,the formers got to their peak 24h while the latters got to their peak 12h ,both of them kept a high level on 48h. Conclusions ：The expression of Cathepsin D and caspase-9 after ischemia-reperfusion is high, Lysosomes may be involved in the process of neuronal apoptosis in the brain after ischemia-reperfusion injury
出处
《现代生物医学进展》
CAS
2010年第5期851-854,共4页
Progress in Modern Biomedicine
