摘要
分别以聚马来松香己二醇酯、马来松香乙二醇丙烯酯聚合物和马来松香乙二醇丙烯酯-丙烯酸共聚物为载体,稀土离子为桥键配离子固定化淀粉酶。测定固定化淀粉酶的性能,探讨固定化酶反应机理。结果表明,稀土离子作为桥键配离子的固定化淀粉酶中,PMG AE Y(Ⅲ)En和PMG A EDy(Ⅲ)En效果较好;最适宜温度均为60℃,最适宜pH值均为6.03;重复使用4次后,活性分别为31.49、29.76mg/g·5min。此外,建立了固定化酶活性与功能高分子微孔面积及酸值间的数学关系式。
With polyhexmethylene maleic rosinate,polyethylene maleic rosinate polymer or its copolymer with acrylic acid as matrix,amylase was immobilized on La（Ⅲ）,Eu（Ⅲ）,Tb（Ⅲ）,Y（Ⅲ） or Dy（Ⅲ） coordinates of the matrix.The performance of the immobilized enzyme and the mechanism of immobilization were investigated.High activities were observed for the Y（Ⅲ） and Dy（Ⅲ） coordinates of the functional polymers.The optimal temperature and pH were both 60 ℃ and 6.03,respectively.The residual activities after used for 4 cycles were 31.49 and 29.76 mg/g·5 min.In addition,the correlation between the activities of the immobilized enzymes and surface area of the matrix as well as pH was established.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第7期199-204,共6页
Food Science
基金
广西科学研究与技术开发基金资助项目(桂科攻0630004-1
桂科攻08395003-9)
关键词
松香基功能高分子
固定化酶
淀粉酶
稀土离子
rosin based functional polymers
enzyme immobilization
amylase
rare earth ion
