摘要
目的探讨羟乙基淀粉130／0．4对内毒素致大鼠急性肺损伤（Au）时Toll样受体4（TLR4）表达的影响。方法雄性SD大鼠30只，体重250～300g，随机分为5组（n=6）。生理盐水对照组（NS组）、ALI组和H1-3组。ALI组、H1组和H2组经右颈内静脉注射内毒素10mg／kg制备大鼠ALI模型，H1组和H2组注射内毒素完毕1min后，右颈内静脉分别输注6％羟乙基淀粉130／0．415和30ml／kg，H3组仅右颈内静脉输注6％羟乙基淀粉130／0．430ml／kg，速率均为0．2ml／min。注射内毒素后6h时处死大鼠取肺，光镜下观察肺组织病理学；采用RT-PCR检测TLR4 mRNA的表达水平，Western bloting法检测肺组织TLR4蛋白的表达水平。结果与NS组相比，ALI组TLR4 mRNA和蛋白的表达上调（P〈0．05），H3组差异无统计学意义（P〉0．05）；与ALI组相比，H1组和H2组TLR4 mRNA和蛋白的表达下调（P〈0．05）；H1组和H2组TLR4 mRNA和蛋白的表达比较差异无统计学意义（P〉0．05）。病理结果显示：H1组和H2组肺损伤程度较ALI组明显减轻。结论羟乙基淀粉130／0．4可能通过抑制TLR4表达上调，减轻炎性反应，从而减轻内毒素致大鼠ALI。
Objective To investigate the effect of hydroxyethyl starch （HES） 130/0.4 on Toll-like receptor 4 （TLR4） mRNA and protein expression in the lung tissue in a rat model of acute lung injury （ALI） induced by lipopolysaccharide （LPS）. Methods Thirty pathogen-free male SD rats weighing 250-300 g were randomly divided into 5 groups （ n = 6 each） ： group Ⅰ normal control （group C） ; group Ⅱ ALI; group Ⅲ and ⅣHES 15 ml/kg or 30 ml/kg ＋ALI and group Ⅴ HES 30 ml/kg. The animals were anesthetized with intraperitoneal 10% chloral hydrate 300 mg/kg. The right internal jugular vein was cannulated for fluid and drug administration. ALI was induced by LPS 10 mg/kg iv in group Ⅱ ,Ⅲ and Ⅳ. HES 130/0.4 was infused iv at 0.2 ml/min at 1 min after LPS administration in group Ⅲ , Ⅳ and Ⅴ . The animals were killed at 6 h after IP LPS and the lungs were immediately removed for microscopic examination and detection of TLR4 mRNA and protein expression by RT-PCR and Western blotting. Results The TLR4 mRNA and protein expression was significantly higher in LPS group than in group C. HES 130/0.4 15 and 30 ml/kg significantly reduced the LPS-imluced increase in TLR4 mRNA and protein expression in the lung tissue. There was no significant difference in TLR4 mRNA and protein expression between the two ALI＋ HES groups （ Ⅲ , Ⅳ ）. Conclusion HES 130/0.4 can inhibit ALI-induced ALI by depressing the TLR4 expression in the lung.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第2期202-204,共3页
Chinese Journal of Anesthesiology
基金
上海市卫生局百人计划基金（03-77-20）
上海交通大学医学院麻醉学重点学科
