摘要
目的μ阿片受体A118G基因（OPRM1 A118G基因）多态性对妇科手术患者电刺激痛敏感性的影响。方法择期拟行子宫肌瘤剔除术或子宫全切术患者152例，汉族，年龄20～50岁，体重指数19～21kg／m^2，ASAⅠ或Ⅱ级，根据基因型分为野生型纯合子组（A／A组）、突变型杂合子组（A／G组）和突变型纯合子组（G／G组）。采用电刺激测定患者痛阈和耐痛阚。使用聚合酶链反应．限制性片段长度多态性技术检测OPRM1 A118G基因多态性。结果A／A组76例，A／G组56例，G／G组20例；G等位基因频率为31．6％。三组间痛阈差异无统计学意义（P〉0．05）；G／G组耐痛阈低于A／A组和A／G组（P〈0．05）；A／A组和A／G组耐痛阈差异无统计学意义（P〉0．05）。结论OPRM1 A118G基因突变可导致妇科手术患者耐痛阈降低，对痛阈无影响。
Objective To determine the effects of A118G polymorphism in μ opioid receptor gene on pain sensitivity in patients undergoing gynecological operation. Methods One hundred and fifty-two gynecological patients, aged 20-50 yr, ASA Ⅰ or Ⅱ , with body mass index 19-21 kg/m^2,Han nationality, were divided into 3 groups according to the genotype： group A/A （wild-type homozygous） ; group A/G （mutant heterozygous） and group G/G （mutant homozygous）. Pain threshold and pain tolerance threshold were measured using electrical stimulation. Genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP） for A118G polymorphism in μ opioid receptor gene. Results Genotyping analysis revealed that genotype distribution of A118G polymorphism was A/A 76 cases, A/G 56 cases and G/G 20 cases. The G allele frequency was 31.6%. There was no difference in pain threshold among the 3 groups （P 〉 0.05）. But the pain tolerance threshold was significantly lower in group G/G than in group A/A and group A/G （P 〈 0.05）. Conclusion The A118G allele gene mutation in μ opioid receptor gene decreases pain tolerance threshold but has no effects on pain threshold in patients undergoing gynecological operation.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第2期159-161,共3页
Chinese Journal of Anesthesiology
关键词
受体
阿片样
μ
多态性
单核苷酸
痛阈
妇科外科手术
Receptors, opioid, mu
Polymorphism, single nucleotide
Pain threshold
Gynecologic surgical procedures
