摘要
目的：研究溶剂浓度、溶剂倍量、提取时间和提取温度对当归四逆汤复方提取的影响，并通过药理实验筛选出最佳提取工艺。方法：设计乙醇浓度、溶剂倍量、提取时间和提取温度4个因素的正交实验，以药理实验缺氧耐受性为指标。结果：小鼠的缺氧耐受性实验结果显示：小鼠存活时间以第6组最长，其次为第2、第4第3组，存活时间最短的是第8组。结论：当归四逆汤的最佳提取工艺为以4倍量70％乙醇回流提取2小时。
Objective： To investigate the flux concentration, times the volume of solvent, extraction time and extraction temperature on extraction of Danggui Sini Decoction compound the impact of, and pharmacological experiments in mice were selected to do the best extraction process. Method： Consider the ethanol concentration, times the volume of solvent, extraction time and extraction temperature of four orthogonal experimental design factors, through the hypoxic tolerance of the mouse experiment, the data recording and analysis of experimental results obtained. Result： Hypoxia tolerance in mice experimental observation： the first group： 10.07±1.25min Second group： 11.57± 1.36min Third group： 11.12±1.43min fourth group： 11 ± 1.34min fifth group： 10.87±1.02min sixth group： 14.67 ± 0.72min seventh group： 10.35±1.25min eighth group： 9.23± 1.07min ninth group： 9.93±0,87min. Conclusion： Danggui Sini Decoction extraction process for the best 4 times to 70% ethanol extraction for 2 hours.
出处
《中医临床研究》
2010年第6期106-107,共2页
Clinical Journal Of Chinese Medicine
关键词
当归四逆汤复方
正交实验
缺氧耐受性实验
Danggui Sini Decoction compound
orthogonal experiments
hypoxia tolerance test
