摘要
利用ＴｒｉｔｏｎＸ－１００及其联合（ＮＨ４）２ＳＯ４的抽提技术与ＣｏｏｍａｓｉｅｂｌｕｅＲ２５０染色、免疫酶标技术相结合，体外实验比较研究人肝癌细胞系（ＨｅｐＧ２）细胞骨架网络的分布构像及其中间纤维蛋白构型。结果：人肝癌细胞系（ＨｅｐＧ２）显示其细胞骨架网络的分布构像及其中间纤维对Ｖｉｍｅｎｔｉｎ、Ｋｅｒａｔｉｎ两种抗体均呈现阳性反应。结论：恶性肿瘤细胞的中间纤维蛋白构型可能具有异质性的蛋白分子共表达，这对仅以ＴｒｉｔｏｎＸ－１００加（ＮＨ４）２ＳＯ４的抽提技术进行恶性肿瘤细胞中间纤维蛋白构型分析，是一个必需考虑的问题。
Human hepatocellular carcinoma cell line (Hep G2) was extracted with triton X-100 or triton X-100 and (NH 4) 2SO 4, and then stained with Coomasie blue R 250 or labelled by immunoenzymatic technique, to show the feature of the cytoskeleton network and its componets of intermediate filament. The cell (Hep G2) was showed the feature of cytoskeleton network and its components of intermediate filament which were possitive for both Keratin and Vimentin antibodies. Conclusion: the components of intermediate filament protein of malignant cells might be heterogenous. It suggested that, in analysing the components of intermediate filament protein of malignant tumor cells which were extracted with triton X-100 and (NH 4) 2 SO 4 in combination, the heterogenous proteins co-expression must be taken into consideration
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1998年第2期18-21,共4页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学基金
关键词
肝癌
细胞骨架
中间纤维
蛋白质
hepatocellular neoplasm
\ cytoskeleton
\ intermediate filament
\ protein
